PLGA (poly(lactic-co-glycolic acid)) is a biodegradable and biocompatible polymer, FDA-approved for biomedical applications. Upon administration, the PLGA polymer degrades through hydrolysis of its ester backbone into non-toxic byproducts. For drug-loaded particles, the drug molecules are gradually released, and this release rate can be fine-tuned by selecting different types of PLGA and adjusting the encapsulation process.

Factors that influence the degradation and drug release rates include:

• Lactide to glycolide (L/G) ratio: a 50:50 L/G ratio exhibits the fastest drug release
• Molecular weight: Higher molecular weights result in slower release
• Terminal groups: Carboxyl-terminated PLGA polymer provide quicker release compared to ester-terminated PLGA

TM="Degradex" Green Fluorescent PLGA nanoparticles are ideal for a wide range of biomedical and pharmaceutical R&D applications, including:

• Controlled drug release: The gradual degradation of PLGA allows for sustained release of encapsulated drug molecules, improving therapeutic efficacy and reducing dosing frequency.
• Bioavailability enhancement: PLGA nanoparticles can improve the solubility and stability of poorly water-soluble drugs, enhancing their bioavailability.
• Drug targeting: The nanoparticle structure enables targeted drug delivery to specific tissues or cells, potentially reducing side effects and improving treatment outcomes.
• API stabilization: Encapsulation in PLGA nanoparticles can protect sensitive active pharmaceutical ingredients (APIs) from degradation.
• Drug attachment and delivery: The nanoparticles can act as carriers, allowing drug molecules to be attached to their surface for efficient delivery.
• Imaging and cell tracking: Fluorescent nanoparticles can be utilized for imaging, fluid tracing, cell tracking, phagocytosis studies, and fluorescence microscopy. They are particularly effective in drug delivery applications and diagnostic R&D, particularly for detecting binding events or enhancing signals.

TM="Degradex" Green Fluorescent PLGA nanoparticles are made with poly(D,L-lactide-co-glycolide) with an L/G ratio of 50/50 and a MW of 30,000.

Key Features:

Average diameter: 500nm +/- 100nm
Standard deviation: <50%
Fluorescence: Green
Excitation/Emission: 460/500 nm

Packaged as a lyophilized powder, Degradex nanoparticles are designed to be reconstituted in an aqueous medium, such as deionized water or buffer, for common applications. It is crucial to ensure the particles are well suspended as a single-particle suspension without aggregation before use.

Reconstitution Instructions:

• Remove the product from the freezer.
• Allow it to reach ambient temperature.
• Add deionized water or buffer to the container.
• Gently mix the contents to ensure the particles are well suspended; vortexing or sonication may be necessary.

Nanoparticle size can be measured using analytical methods such as dynamic light scattering (DLS).

Product of Phosphorex, Inc.