Monophosphoryl lipid A (MPLA) is either extracted from bacterial lipid A or by chemical synthesis.

Vaccination is well-accepted as an effective method to prevent infections by mounting pathogen-specific immune responses prior to the infection. Usually, immunization with vaccine antigens alone is not able to induce robust or long-lasting immune responses — resulting in failure of protective immunity against infections. Thus, adjuvants are required to enhance cellular or humoral immune responses upon immunization. Because vaccine adjuvants using Lipid A have proven to be safe and effective in inducing Th-1 type immune responses to heterologous proteins in animal and human vaccines, Avanti developed Phosphorylated HexaAcyl Disaccharide (PHAD^™), the first fully synthetic monophosphoryl Lipid A available for use as an adjuvant research.

MPLA PHAD^™ has been used:

• as a component of cobalt porphyrin-phospholipid (Co-PoP) liposomes for the immunization of mice with membrane proximal external region (MPER) of the gp41 envelope protein
• as an adjuvant along with dimethyldioctadecylammonium bromide(DDA) for C. muridarum recombinant membrane protein based multi-subunit vaccine
• as toll-like receptor-4 (TLR4) agonist adjuvant for respiratory syncytial virus (RSV) fusion (F) protein FI-RSV vaccine

Monophosphoryl lipid A (MPLA) is a natural agonist for the toll-like receptor-4 (TLR4). It is useful as an adjuvant in immunization. MPLA is a safe prophylactic agent and has immunotherapeutic applications. MPLA used in vaccination improves B cell and T cell-mediated immunity.

2 mL Amber Glass Crimp Cap Vial (699800P-1mg)

2 mL Amber Glass Crimp Cap Vial (699800P-5mg)

Soluble in ethanol at 1mg/mL, soluble in DMSO at 5mg/mL, soluble in Chloroform:Methanol:Water at 5mg/mL, insoluble in water (aqueous buffers, serum etc.); however, it can be dispersed in an aqueous medium using a suitable emulsifier or detergent. This species is soluble in chloroform (at least up to 50 mg/mL) and chloroform:methanol solvent systems, however gentle heating and sonication may be necessary to aid in dissolution. Dropwise additions of methanol or water may aid dissolution in chloroform at higher concentrations. If using in combination with other lipids, dissolve this lipid separately and then combine dissolved lipids in their respective solvent solutions.

For R&D use only. Not for drug, household, or other uses.

Avanti Research is a trademark of Avanti Polar Lipids, LLC

PHAD is a trademark of Avanti Polar Lipids, LLC