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1.50447

SeQuant® ZIC®-HILIC 200Å (3.5 µm) HPLC Columns

L × I.D. 100 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

Synonym(s):

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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About This Item

UNSPSC Code:
41115709
NACRES:
NB.21
Particle size:
3.5 μm
Matrix active group:
ZIC-HILIC bonding phase
Pore size:
200 Å pore size
Matrix:
fully porous particle
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Product Name

SeQuant® ZIC-HILIC™ 3.5µm,200Å 100 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC

material

PEEK-lined stainless steel column

Quality Level

100

agency

suitable for USP L114

product line

SeQuant®

feature

endcapped: no

parameter

550 bar pressure, 70 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

10 cm × 2.1 mm

matrix

fully porous particle

matrix active group

ZIC-HILIC bonding phase

particle size

3.5 μm

pore size

200 Å pore size

operating pH

2-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

General description

SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.40

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

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Articles

Selectivity Examination of Stationary Phases for HILIC

This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.

Liquid chromatography-mass spectrometry metabolite library for metabolomics: Evaluating column suitability using a scoring approach
Diamantidou D, et al.
Journal of Chromatography A, 1690, 463779-463779 (2023)
Dimitra Diamantidou et al.
Journal of chromatography. A, 1690, 463779-463779 (2023-01-22)
Untargeted metabolomic studies require an extensive set of analyte (metabolic) information to be obtained from each analyzed sample. Thus, highly selective, and efficient analytical methodologies together with reversed-phase (RP) or hydrophilic interaction liquid chromatography (HILIC) are usually applied in these

Global Trade Item Number

SKUGTIN
150447000104027269134297