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About This Item
UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
5 μm
Matrix active group:
C8 bonding phase
Pore size:
60 Å pore size
Matrix:
fully porous particle
Product Name
LiChrospher® RP-select B (5µm) Hibar® RT 250-4.6, suitable for HPLC
material
stainless steel column
Quality Level
agency
suitable for USP L7
product line
LiChrospher®
feature
endcapped: no
extent of labeling
11.5% Carbon loading
parameter
400 bar pressure, 60 °C max. temp.
technique(s)
HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium)
column L × I.D.
25 cm × 4.6 mm
surface area
350 m2/g
matrix
fully porous particle
matrix active group
C8 bonding phase
particle size
5 μm
pore size
60 Å pore size
operating pH range
2-7.5
separation technique
reversed phase, supercritical fluid (SFC)
storage temp.
no temp limit
General description
HPLC column
LiChrospher® RP-select B is a versatile traditionally produced spherical silica gel carriers with reversed phase properties and with the hydrophobicity of a C-8 phase. This carrier is very well suited for the separation of basic compounds, but with still good properties for the determination of neutral and acidic compounds. This sorbent is packed into a Hibar 100-4.6 HPLC column. For mounting of a LiChroCART 4-4 guard column, the guard column holder 1.51487 has to be used.
Application
- Simultaneous quantification of delta-9-THC, THC-acid A, CBN and CBD in seized drugs using HPLC-DAD: This study utilizes high-performance liquid chromatography with diode-array detection (HPLC-DAD), employing a LiChrospher® RP-select B column to effectively separate and quantify cannabinoids in forensic samples, highlighting its application in legal and forensic settings (Ambach et al., 2014).
- Structural characterization of stress degradation products of Darunavir: Employing LC-MS/MS alongside HPLC, this research underscores the utility of the LiChrospher® RP-select B column in the stability analysis of HIV medications, crucial for ensuring drug safety and efficacy (Rao et al., 2014).
- RP-HPLC separation and characterization of unknown impurities of Darunavir: This study demonstrates the column′s role in identifying and quantifying impurities in HIV-protease inhibitors, contributing to the pharmaceutical industry′s quality control measures (Nageswara Rao et al., 2013).
- Quantification of pralidoxime in urine by ion pair chromatography: The LiChrospher® RP-select B column is applied in a biomedical context to monitor therapeutic agents in clinical settings, supporting patient treatment regimes (John et al., 2012).
- Liquid chromatography/electrospray tandem mass spectrometry method for determination of cefuroxime: This analytical method facilitates the study of pharmacokinetics in clinical pharmacology, using the LiChrospher® RP-select B column for precise drug monitoring in plasma (Partani et al., 2010).
Analysis Note
Pressure: ≤ 90 bar
Theoretical Plates (N/m) (Toluene): ≥ 50000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.0 - 2.0
Theoretical Plates (N/m) (Toluene): ≥ 50000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.0 - 2.0
Legal Information
Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 1505750001 | 04027269150617 |
| 1505751003 | 04061838831453 |