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Supelco

Purospher® STAR RP-18 endcapped (2 µm)

L × I.D. 30 mm x 2.1 mm Hibar® HR UHPLC column

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Synonym(s):
Purospher® STAR RP-18 endcapped (2µm) Hibar® HR 30-2.1

material

stainless steel column

Quality Level

100

Agency

suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

17% Carbon loading

parameter

1000 bar pressure
65 °C max. temp.

technique(s)

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

3 cm × 2.1 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

2 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase
supercritical fluid (SFC)

storage temp.

no temp limit

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General description

Purospher® STAR RP-18 endcapped, 2 µm UHPLC columns are designed for use in UHPLC Applications and instruments providing full UHPLC resolution and highest separation efficiency′s at maximum pressure of 1000 bar.Purospher® STAR RP-18 endcapped, 2 µm UHPLC columns allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Purospher® STAR RP-18 endcapped, 2 µm Hibar® HR 30-2.1 is a ready to use UHPLC column suitable for connection to any UHPLC system providing a pressure stability of 1000 bar.The column dimension of 30 mm length x 2.1 mm I.D. is the optimal choice for rapid UHPLC separation of complex samples allowing significant solvent savings and improved sensitivity. A 2.1 mm I.D. column enables a more sustainable separations in comparison to 4, 4.6 or 3 mm I.D. column due to the use of significant less solvent for the chromatographic separation.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 90 bar
Theoretical Plates (N/m) (1,2-Benzanthracene): ≥ 170000
Symmetry (1,2-Benzanthracene): 0.8 - 1.4
Capacity factor (1,2-Benzanthracene): 7.0 - 10.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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