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1.50662

SeQuant® ZIC®-cHILIC 100Å (3 µm) HPLC Columns

L x I.D. 150 mm x 4.6 mm HPLC Column, PEEK-lined stainless steel

Synonym(s):

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52
Particle size:
3 μm
Matrix active group:
ZIC-cHILIC bonding phase
Pore size:
100 Å pore size
Matrix:
fully porous particle
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Product Name

SeQuant® ZIC®-cHILIC 3µm,100Å 250 x 4.6 mm, PEEK-lined stainless steel column, suitable for HPLC

material

PEEK-lined stainless steel column

Quality Level

100

product line

SeQuant®

feature

endcapped: no

parameter

500 bar pressure, 70 °C max. temp.

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

25 cm × 4.6 mm

matrix

fully porous particle

matrix active group

ZIC-cHILIC bonding phase

particle size

3 μm

pore size

100 Å pore size

operating pH

1-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

General description

SeQuant® ZIC®-cHILIC is designed for excellent HPLC and LC-MS of polar hydrophilic compounds. This zwitterionic stationary phase with phosphorylcholine functional group provides you with complementary selectivity for easier method development for substances that have been difficult to separate by previous types of HPLC columns operated in reversed-phase or HILIC mode.The column dimension of 250 mm length x 4.6 mm ID is the optimal choice for the separation of complex samples using a standard HPLC Instrument.SeQuant® ZIC®-cHILIC HPLC columns provide complementary selectivity to optimize separations of polar hydrophilic compounds. The columns carry a novel densely bonded, 1:1 charge-balanced phosphorylcholine functional group. As with all true zwitterionic stationary phases, straightforward and reproducible separations are achieved by hydrophilic partitioning combined with weak ionic interactions.The more accessible positive charges of SeQuant® ZIC®-cHILIC columns enable selectivity tuning in chromatography through altered interaction with anionic and cationic molecular moieties. This can be favorable in several situations, for example in the separation of labile metal complexes or highly polar and multiply charged aminoglycosides. Their extraordinary stability and minimized bleed also make SeQuant® ZIC®-cHILIC columns the preferred choice for many LC-MS applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Analysis Note

Pressure: ≤ 90 bar
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.25

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

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Protocols

Global Trade Item Number

SKUGTIN
150662000104061838831767