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Merck
CN

1.51914

Supelco

Purospher® STAR RP-18 endcapped (5 µm) HPLC Columns

L × I.D. 125 mm x 4.6 mm Hibar® RT HPLC Column

Synonym(s):

Purospher® STAR RP-18 endcapped (5µm) Hibar® RT 125-4.6

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

Key Documents

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Product Name

Purospher® STAR RP-18 endcapped (5 µm), L × I.D. 125 mm x 4.6 mm Hibar® RT HPLC column

material

stainless steel column

Agency

suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

17% Carbon loading

parameter

400 bar pressure
65 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

column L × I.D.

12.5 cm × 4.6 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase
supercritical fluid (SFC)

storage temp.

no temp limit

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General description

Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher® STAR RP-18 endcapped, 5 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher® STAR RP-18 endcapped, 5 µm Hibar® RT 125-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The column dimension of 125 mm length x 4.6 mm I.D. is the optimal choice for the high resolution HPLC separation of complex samples.The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART™ 4-4 part number 1.50250.0001. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART™ cartridges, part number 1.51487.0001 can be used.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Regulatory Information

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Articles

Determination of Impurities in Clozapine API

HPLC-UV quantitative analysis of four clozapine-related substances in clozapine API as per Ph. Eur. Monograph using Purospher® Star RP-18 endcapped column.

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