1.52258
Chromolith® WP 300 Protein A (monolithic) HPLC Columns
L × I.D. 25 mm x 4.6 mm HPLC Column
Synonym(s):
Chromolith® WP 300 Protein A 25-4.6 HPLC column
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About This Item
UNSPSC Code:
41115700
NACRES:
SB.52
Matrix active group:
Protein A bonding phase
Pore size:
2 μm macroporosity, 30 nm mesoporosity
Matrix:
monolithic silica
Product Name
Chromolith® WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column
material
PEEK column
product line
Chromolith®
feature
endcapped: no
packaging
1 ea of
parameter
2 - 45 °C temp. range, 2-45 °C temp. range, 200 bar pressure
technique(s)
HPLC: suitable, LC/MS: suitable
column L × I.D.
2.5 cm × 4.6 mm
surface area
120 m2/g
matrix
monolithic silica
matrix active group
Protein A bonding phase
pore size
2 μm macroporosity, 30 nm mesoporosity
operating pH range
1.5-7.5
operating pH
1.5-7.5
separation technique
affinity
storage temp.
2-8°C
General description
Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.
Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.
Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.
Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
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