1.52260
Chromolith® WP 300 RP-4 (monolithic) HPLC Columns
L × I.D. 100 mm x 4.6 mm HPLC Column
Synonym(s):
Chromolith® WP 300 RP-4 100-4.6 HPLC column
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About This Item
UNSPSC Code:
41115700
NACRES:
SB.52
Matrix active group:
C4 bonding phase
Pore size:
2 μm macroporosity, 30 nm mesoporosity
Matrix:
monolithic silica
Product Name
Chromolith® WP 300 RP-4, L × I.D. 100 mm x 4.6 mm HPLC column
material
PEEK column
Quality Level
product line
Chromolith®
feature
endcapped: no
packaging
1 ea of
extent of labeling
3-4% Carbon loading
parameter
200 bar pressure, 60 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)
column L × I.D.
10 cm × 4.6 mm
matrix
monolithic silica
matrix active group
C4 bonding phase
pore size
2 μm macroporosity, 30 nm mesoporosity
operating pH range
1.5-7.5
separation technique
reversed phase
storage temp.
no temp limit
General description
Biotherapeutics, for example, bio-engineered drugs and peptide therapeutics, represent the promise of new medical treatments for the future. Production costs have been falling leading to extremely high demand for suitable analytical methods for process monitoring and quality control of these biomolecules. HPLC is the preferred method of analysis, and it is therefore important to use the right column for these larger molecules. Accurate analysis of proteins, antibodies, and large peptides requires columns with good permeability, along with better mass transfer and selectivity. In order for size-exclusion not to influence the separation, the pore size should be approximately ten times larger than the molecule being analyzed. Chromolith columns have shown great potential and superiority in comparison to standard silica. Chromolith® WP 300 columns are made of monolithic silica with a characteristic bimodal pore structure. Chromolith® WP 300 RP-4 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 30 nm (300 Å), and the surface modification is Butylsilane. Chromolith® WP 300 RP-4 columns fall under the USP L26 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® WP 300 RP-4 columns are suitable for LC-MS applications.The column dimension of 100 mm in length and 4.6 mm I.D. is the best choice for fast separations of Biomolecules on any HPLC System. Chromolith® WP 300 RP-4 100-4.6 HPLC columns enable:
- Completely bioinert column hardware and high biorecovery
- High-speed separations at very low column back-pressure
- High matrix tolerance, allowing time and cost savings with simplified workflows
- Cost-savings due to extended column lifetimes
- Connection of columns, flow gradients and the use of mobile phases with higher viscosity
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
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Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 1522600001 | 04054839085307 |