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Chromolith® WP 300 Protein A (monolithic) HPLC Columns

L × I.D. 25 mm × 2 mm HPLC Column

Synonym(s):

Chromolith® WP 300 Protein A 25-2 HPLC column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
Matrix active group:
Protein A bonding phase
Pore size:
2 μm macroporosity, 30 nm mesoporosity
Matrix:
monolithic silica
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Product Name

Chromolith® WP 300 Protein A, L × I.D. 25 mm × 2 mm HPLC column

Quality Level

100

feature

endcapped: no

parameter

2-45 °C temp. range, 200 bar pressure

technique(s)

HPLC: suitable

column L × I.D.

25 mm × 2 mm

matrix

monolithic silica

matrix active group

Protein A bonding phase

pore size

2 μm macroporosity, 30 nm mesoporosity

operating pH

1.5-7.5

storage temp.

2-8°C

General description

Affinity chromatography is a selective technique that takes advantage of specific molecular interactions, for example, antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith® WP 300 Protein A, 25-2 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
  • Rapid and accurate titer determination across a wide range of injection volumes
  • Consistent binding efficiencies even at high flow rates
  • Expanded column lifetimes


Learn more:
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns

Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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Articles

Monoclonal Antibody Titer Determination Using a Chromolith® WP 300 Protein A Wide Pore Monolithic Silica HPLC Column

Method development for titer determination of three different monoclonal antibodies - cetuximab, trastuzumab, and universal antibody standard using a Chromolith® WP 300 Protein A column.

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Global Trade Item Number

SKUGTIN
152358000104061841125372