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Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor^™ 488

Name: Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor<SUP>™</SUP> 488
Brand: MM

clone 11E6, Upstate^®, from mouse

Synonym(s):

PKB, PKD-alpha, AKT1, Protein Kinase B

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

ALEXA FLUOR^™ 488

Clone:

11E6, monoclonal

Application:

FACS, ICC, IF, WB

Citations:

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Properties

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR^™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

11E6, monoclonal

species reactivity

rat, canine, human, mouse

manufacturer/tradename

Upstate^®

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001014431.1, NM_001014432.1, NM_005163.2

UniProt accession no.

P31749

shipped in

wet ice

target post-translational modification

phosphorylation (pSer473)

Description

General description

60 kDa

Immunogen

KLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473.

Application

Detect phospho-Akt1/PKBα (Ser473) using this Anti-Akt1 Antibody, clone 11E6, Alexa Fluor 488 validated for use in FC, IC, IF & WB.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Biochem/physiol Actions

Recognizes phosphorylatedAkt1/PKBα at Ser473. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473.

Physical form

Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography

Preparation Note

1 year at 4°C from date of shipment

Analysis Note

Routinely evaluated by immunocytochemistry and flow cytometry.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Alexa Fluor is a registered trademark of Molecular Probes, Inc.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c