Flow Cytometry Antibodies

a graphical representation of an antibody’s interaction with antigens, featuring a yellow Y-shaped structure on a purple background with green dots at the tips, and colored lines suggesting different pathways or interactions.

Flow cytometry analysis is a technique commonly used to examine physical and chemical characteristics of cells and particles. Antibodies are used in flow cytometry to help characterize markers on the cell surface since they allow for the detection of specific antigens. These antibodies can be directly conjugated to a fluorophore (direct detection) or paired with a fluorescently labeled secondary antibody (indirect detection). By adding specific fluorophores to specific antibodies, cells can be sorted and analyzed simultaneously.  

For successful flow cytometry assays and reproducible results, antibodies validated for use in flow cytometry and proper controls are essential. We offer high-quality flow cytometry validated primary antibodies, secondary antibodies, and isotype control antibodies to support all your flow cytometry needs.

Need Help Finding the Correct Antibody for Your Application?

Use our Antibody Explorer search tool to view and compare antibodies by clonality, application, species reactivity, conjugate, host species, and form.

For Research Use Only. Not For Use In Diagnostic Procedures.

Unless otherwise stated in the Product(s) specifications, any Antibody product is sold for internal research use only and may not be used for any other purpose, which includes but is not limited to, any commercial, diagnostic, or therapeutic use. Our validation processes pertain only to research uses and do not confirm or assure that our antibodies can be used for any unauthorized uses as set forth herein.

Related Resources

  • Article: Flow Cytometry Guide

    Explore our flow cytometry guide to uncover flow cytometry basics, traditional flow cytometer components, key flow cytometry protocol steps, and proper controls.

  • Article: Key Steps in Flow Cytometry Protocols

    Learn key steps in flow cytometry protocols to make your next flow cytometry experiment run with ease.

  • Article: Multicolor Flow Cytometry

    Uncover tips for multicolor flow cytometry experiments, including the challenges in multicolor flow cytometry, steps for how to build a multicolor flow cytometry panel, tandem dyes, and tools for multicolor assay design.

  • Article: How to Select Flow Cytometry Dyes

    Discover how to select flow cytometry dyes with tips for flow cytometry dye selection including matching fluorescence profiles of fluorophores to the configuration of flow cytometers, the factors that can affect fluorophore performance in a multicolor panel, and helpful tools for simple dye selection.

  • Article: Flow Cytometry Troubleshooting Guide

    Need tips on how to improve your flow cytometry analysis? Check out this flow cytometry troubleshooting guide with possible causes and solutions for most common problems encountered during flow cytometry experiments, including a loss or lack of signal, high background, decreasing acquisition rates, and more.

  • Article: How to Choose a Secondary Antibody

    Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.

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