Key Documents

View All Documentation

203980

α-Bungarotoxin

Name: α-Bungarotoxin
Brand: MM

from Bungarus multicinctus, No contaminants by IEF, lyophilized solid, irreversible binder to motor end-plate acetylcholine receptor, Calbiochem^®

Synonym(s):

α-Bungarotoxin, Bungarus multicinctus

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₃₃₈H₅₂₉N₉₇O₁₀₅S₁₁

CAS Number:

11032-79-4

Molecular Weight:

7984.12

MDL number:

MFCD00007185

UNSPSC Code:

12352200

NACRES:

NA.77

Form:

lyophilized solid

Quality level:

100

Storage condition:

OK to freeze

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

α-Bungarotoxin, Bungarus multicinctus, Blocks neuromuscular transmission by irreversible binding to motor end-plate acetylcholine receptor (K_d = 1 pM to 1 nM) but does not depress acetylcholine release from motor nerve endings.

Quality Level

100

description

Merck USA index - 14, 1488

form

lyophilized solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

solubility

PBS: soluble, water: soluble

shipped in

ambient

storage temp.

−20°C

Description

General description

A polypeptide composed of 74 amino acids containing 5 disulfide bridges. Blocks neuromuscular transmission by irreversible binding to the motor end-plate acetylcholine receptor (K_d = 1 nM to 1 pM) but does not depress acetylcholine release from motor nerve endings. Blocks nicotine-induced augmentation in intracellular Ca²⁺ in PC12 cells (IC₅₀ = 310 nM). Prevents opening of nicotinic receptor-associated ion channels. Reconstitution experiments in Xenopus oocytes have shown the effects of α-Bungarotoxin on neuronal nAChR to be highly specific for the α₇-subtype (IC₅₀ = 1.6 nM), but not for the α₃β₄-subtype (IC₅₀ >3 µM).

Blocks neuromuscular transmission by irreversible binding to motor end-plate acetylcholine receptor (K_d = 1 pM to 1 nM) but does not depress acetylcholine release from motor nerve endings. Blocks nicotine-induced increase of intracellular Ca²⁺ in PC12 cells (IC₅₀ = 310 nM), and prevents opening of nicotinic receptor-associated ion channels. Reconstitution experiments in Xenopus oocytes have shown the effects of α-bungarotoxin on neuronal nAChR to be highly specific for the α₇-subtype (IC₅₀ = 1.6 nM), but not for the α₃β₄-subtype (IC₅₀ >3 µM).

Biochem/physiol Actions

Cell permeable: no

Kd = 1 pM to 1 nM for motor end-plate acetylcholine receptor

Primary Target
Motor end-plate acetylcholine receptor

Product does not compete with ATP.

Reversible: no

Physical form

Supplied as an acetate salt

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Analysis Note

Contaminants: None detectable by IEF

Other Notes

H-Ile-Val-Cys³-His-Thr-Thr-Ala-Thr-Ser-Pro-Ile-Ser-Ala-Val-Thr-Cys¹⁶-Pro-Pro-Gly-Glu-Asn-Leu-Cys²³-Tyr-Arg-Lys-Met-Trp-Cys²⁹-Asp-Ala-Phe-Cys³³-Ser-Ser-Arg-Gly-Lys-Val-Val-Glu-Leu-Gly-Cys⁴⁴-Ala-Ala-Thr-Cys⁴⁸-Pro-Ser-Lys-Lys-Pro-Tyr-Glu-Glu-Val-Thr-Cys⁵⁹-Cys⁶⁰-Ser-Thr-Asp-Lys-Cys⁶⁵-Asn-Pro-His-Pro-Lys-Gln-Arg-Pro-Gly-OH (disulfide bonds: 3 → 23; 16 → 44; 29 → 33; 48 → 59; 60 → 65)

Lopez, M.G., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 14184.
Zhang, Z.W., et al. 1994. Neuron 12, 167.
Bambrick, L.L., and Gordon, T. 1992. J. Physiol.449, 479.
Lin, S.R., and Chang, C.C. 1992. Biochim. Biophys. Acta1159, 255.
Motomura, M., et al. 1992. Neurosci. Lett.143, 139.
Sorenson, E.M., and Chiappinelli, V.A. 1992. J. Comp. Neurol.323, 1.
Ruan, K.H., et al. 1990. Proc. Natl. Acad. Sci. USA87, 6156.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c