Name: Human Collagen Type IV
Brand: MM

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About This Item

CAS Number:

9007-34-5

MDL number:

MFCD00130825

UNSPSC Code:

12352202

NACRES:

NA.77

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Product Name

Collagen, Type IV, Human Placenta, (100 μg), solution, purified by (Highly purified), suitable for cell culture, Native protein

biological source

human placenta

Quality Level

100

description

Merck USA index - 14, 2480

Assay

≥90% (SDS-PAGE)

form

solution

purified by

(Highly purified)

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

concentration

(100 μg)

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

−20°C

General description

Collagen Type IV is a primary component of the basement membrane (BM). It is a specialized sheet-like extracellular matrix that surrounds specific cell types such as skeletal muscle cells, smooth muscle cells, heart muscle cells, and adipocytes in multicellular tissues. Collagen type IV is characterized as a nonfibril-forming collagen and is composed of six genetically distinct α-chain polypeptides. These chains have the potential to form 56 different combinations, resulting in various homotrimers. Through self-assembly, collagen type IV constructs organized networks that play a crucial role in maintaining the stability and assembly of the basement membrane (BM). Its presence is essential for the structural integrity and functionality of the BM.

Application

The human placental collagen type IV (Sigma Aldrich 234154) is used:

as a coating substrate for cell culture treated flasks to promote the growth and adhesion of CuFi-1 cells, providing reliable conditions for cell line propagation and maintenance
to coat transwell inserts, providing a suitable substrate for the growth and formation of monolayers in intestinal organoid culture for trans epithelial electrical resistance (TEER) measurement and drug treatments
to coat inserts for two-dimensional monolayer culture

Biochem/physiol Actions

Collagen Type IV interacts with numerous binding partners. Type IV collagen forms a structural scaffold within the basement membrane (BM) along with other macromolecules like laminins, heparan sulfate proteoglycans, fibronectin, and entactin. This complex network regulates the interaction between the BM and adhering cells, providing critical support and functionality. Thereby, regulates cell adhesion and migration.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In sterile 2 mM HCl.

Other Notes

Miller, E.J., and Gay, S. 1987. Methods Enzymol. 144, 3.
Klasson, S.C., et al. 1986. Coll. Relat. Res.6, 397.
Glanville, R.W., et al. 1979. Eur. J. Biochem.95, 383.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)