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34321-M

FITC, Isomer I

Synonym(s):

FITC, Isomer I, Fluorescein Isothiocyanate, Isomer I

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About This Item

Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
3326-32-7
Molecular Weight:
389.38
UNSPSC Code:
41116133
MDL number:
MFCD00005063
NACRES:
NA.41
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assay

≥95% (TLC)

Quality Segment

100

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

color

orange-red

solubility

DMSO: 5 mg/mL, DMF: soluble, aqueous buffer, pH ≥ 9.0: soluble

storage temp.

2-8°C

SMILES string

S=C=Nc1cc2c(cc1)C3(OC2=O)c4c(cc(cc4)O)Oc5c3ccc(c5)O

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

General description

A fluorescent labeling agent used to label antibodies, lectins, toxins, various proteins, and oligopeptides with fluorescein. FITC-antibody conjugates are useful in many applications, including flow cytometry and immunohistochemistry (excitation maximum = 492 nm; emission maximum = 520 nm). λ max: 492 ± 3 nm (pH 9). Fluorescence EMmax: 520 ± 3 nm (pH 9).
Popular derivative of fluorescein. Can be used to label antibodies and other proteins.

Preparation Note

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Cherry, N.B., et al. 1965. Stain Technol. 44, 179.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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