Antibody set containing 10 µl of each of the following antibodies:



Anti-G_iα-1 (Cat. No. 371720)

Anti-G_iα-1 and G_iα-2 (Cat. No. 371723)

Anti-G_iα-3 (Cat. No. 371729)

Anti-G_iα-3 and G_oα (Cat. No. 371726)

Anti-G_sα (Cat. No. 371732)

Anti-G-protein β-subunit (Cat. No. 371738)

Anti-G_qα and G₁₁α (Cat. No. 371751)



Useful for identifying G-protein subunits.

Contains 10 µl of each of the following antibodies: Anti-G_iα-1 (Cat. No. 371720), Anti-G_iα-1 and G_iα-2 (Cat. No. 371723), Anti-G_iα-3 and G_oα (Cat. No. 371726), Anti-G_iα-3 (Cat. No. 371729), Anti-G_sα (Cat. No. 371732), Anti-G_q- and G₁₁α (Cat. No. 371751), and Anti-Gβ-Subunit (Cat. No. 371738). Each antibody in the set is sufficient for one Western blot at 1:1000.

See individual antibody descriptions for more details.

This G-Protein Subunit Antibody Set is validated for use in Immunoblotting.

G-Protein Subunit Antibody Set. Contains 10 µl of each of Anti-Giα-1, Anti-Giα-1 and Giα-2, Anti-Giα-3 and Goα, Anti-Giα-3, Anti-Gsα, Anti-Gq- and G11α, and Anti-Gβ-Subunit.

Immunoblotting (1:1000, all antibodies)

Toxicity: Standard Handling (A)

Each antibody is supplied in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

Following initial, aliquot and freeze (-70°C).

Mumby, S., et al. 1988. J. Biol. Chem.263, 2020.
Yatani, A., et al. 1988. Nature336, 680.
Mumby, S.M., et al. 1986. Proc. Natl. Acad. Sci. USA83, 265.

The specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes encoding each subunit. For immunoblotting: 10 µg purified plasma membranes from rat adipocytes were loaded into each lane and resolved on a 12% acrylamide mini-gel (10 cm x 10 cm). The proteins were transferred to PVDF membranes on a semi-dry blotter and blocked in low detergent blotto (5% milk protein) for 1 hr. The blots were transferred to designated vessels containing the specific antibody diluted in low detergent blotto at 1:1000. Each blot was incubated on a rocker at room temperature with its specific antibody for 2 h, followed by 4 washes with low detergent blotto. Goat anti-rabbit IgG labeled with ¹²⁵I (approximately 5µCi/µg IgG) was diluted to 500,000 cpm/ml in low detergent blotto, followed by several washes to remove milk proteins. The blots were exposed to X-ray film with intensifying screens for the indicated times. Antibodies should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany