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Merck
CN

401002

Iberiotoxin, Buthus tamulus, Recombinant, E. coli

Synonym(s):

Iberiotoxin, Buthus tamulus, Recombinant, E. coli, rlbTx, Pyr-FTDVDCSVSKECWSVCKDLFGVDRGKCMGKKCRCYQ

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About This Item

CAS Number:
129203-60-7
UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
MFCD00131365

Key Documents

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SMILES string

S1SCC(C(=O)NC(C(=O)NC(C1)C(=O)NC(Cc9ccc(cc9)O)C(=O)NC(CCC(=O)N)C(=O)O)CCCN\C(=N\[H])\N)NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C2NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(CSSC2)NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C6NC(=O

InChI

1S/C179H274N50O55S7/c1-87(2)65-111-155(261)208-112(66-93-33-15-13-16-34-93)146(252)195-77-133(239)225-139(88(3)4)172(278)213-117(71-136(244)245)158(264)199-100(44-31-62-190-178(186)187)144(250)193-75-131(237)198-102(40-22-27-58-181)148(254)219-124(167(273)205-109(56-64-285-12)145(251)194-76-132(238)197-101(39-21-26-57-180)147(253)200-105(43-25-30-61-184)151(257)220-123-81-286-288-83-125(221-152(258)106(203-166(123)272)45-32-63-191-179(188)189)169(275)210-113(68-95-46-48-97(234)49-47-95)156(262)206-110(177(283)284)50-53-129(185)235)82-287-291-86-128(168(274)202-104(42-24-29-60-183)150(256)212-116(70-135(242)243)159(265)207-111)224-175(281)142(91(9)10)228-164(270)121(79-231)216-157(263)115(69-96-74-192-99-38-20-19-37-98(96)99)211-170(276)126-84-289-290-85-127(171(277)217-122(80-232)165(271)227-141(90(7)8)174(280)218-120(78-230)163(269)201-103(41-23-28-59-182)149(255)204-108(154(260)222-126)52-55-134(240)241)223-160(266)118(72-137(246)247)214-173(279)140(89(5)6)226-162(268)119(73-138(248)249)215-176(282)143(92(11)233)229-161(267)114(67-94-35-17-14-18-36-94)209-153(259)107-51-54-130(236)196-107/h13-20,33-38,46-49,74,87-92,100-128,139-143,192,230-234H,21-32,39-45,50-73,75-86,180-184H2,1-12H3,(H2,185,235)(H,193,250)(H,194,251)(H,195,252)(H,196,236)(H,197,238)(H,198,237)(H,199,264)(H,200,253)(H,201,269)(H,202,274)(H,203,272)(H,204,255)(H,205,273)(H,206,262)(H,207,265)(H,208,261)(H,209,259)(H,210,275)(H,211,276)(H,212,256)(H,213,278)(H,214,279)(H,215,282)(H,216,263)(H,217,277)(H,218,280)(H,219,254)(H,220,257)(H,221,258)(H,222,260)(H,223,266)(H,224,281)(H,225,239)(H,226,268)(H,227,271)(H,228,270)(H,229,267)(H,240,241)(H,242,243)(H,244,245)(H,246,247)(H,248,249)(H,283,284)(H4,186,187,190)(H4,188,189,191)

InChI key

VDNVVLOBNHIMQA-UHFFFAOYSA-N

assay

≥98% (HPLC)

form

lyophilized solid (Salt-free)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Target IC50: 250 pM against high conductance Ca2+-activated K+ channels
potently inhibits Ca2+-activated K+ channels expressed in Xenopus oocytes
Cell permeable: no
Primary Target
High conductance Ca2+-activated K+ channels
Product does not compete with ATP.
Reversible: yes

Disclaimer

Toxicity: Harmful (C)

General description

Recombinant Buthus tamulus iberiotoxin expressed in E. coli. A single polypeptide chain of 37 amino acids that displays about 68% sequence homology with charybdotoxin (ChTx). Acts as a selective, reversible blocker of high conductance, Ca2+-activated K+ channels (PK,Ca). rIbTx does not block other types of voltage-dependent ion channels, especially other types of K+ channels that are sensitive to inhibition by ChTx. Shown to block PK,Ca in excised membrane patches from bovine aortic smooth muscle, acting exclusively at the outer face of the channel (IC50 = 250 pM). Causes a decrease in probability of channel opening and the channel open mean time. rIbTx appears to modulate ChTx binding by an allosteric mechanism.

Other Notes

Pyr-Phe-Thr-Asp-Val-Asp-Cys⁷-Ser-Val-Ser-Lys-Glu-Cys¹³-Trp-Ser-Val-Cys¹⁷-Lys-Asp-Leu-Phe-Gly-Val-Asp-Arg-Gly-Lys-Cys²⁸-Met-Gly-Lys-Lys-Cys³³-Arg-Cys³⁵-Tyr-Gln-OH (disulfide bonds: 7 →28; 13 → 35; 17 → 33)
Vanelli, G., et al. 1994. J. Appl. Physiol.76, 1098.
Garcia, M.L., et al. 1991. J. Bioeng. Biomembr.23, 615.
Galvez, A., et al. 1990. J. Biol. Chem. 265, 11083.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 3 months at -20°C or up to 2 weeks at 4°C.
Reconstitute in Tris-buffered saline (100 mM NaCl, 10 mM Tris-HCl, pH 7.5) containing 0.1% BSA.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

Certificates of Analysis (COA)

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Hong-Bo Zhao et al.
Communications biology, 4(1), 24-24 (2021-01-06)
Inner hair cell (IHC) ribbon synapses are the first synapse in the auditory system and can be degenerated by noise and aging, thereby leading to hidden hearing loss (HHL) and other hearing disorders. However, the mechanism underlying this cochlear synaptopathy

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