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401002

Sigma-Aldrich

Iberiotoxin, Buthus tamulus, Recombinant, E. coli

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Synonym(s):
Iberiotoxin, Buthus tamulus, Recombinant, E. coli, rlbTx, Pyr-FTDVDCSVSKECWSVCKDLFGVDRGKCMGKKCRCYQ
CAS Number:
129203-60-7
MDL number:
MFCD00131365

Assay

≥98% (HPLC)

Quality Level

100

form

lyophilized solid (Salt-free)

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

−20°C

InChI

1S/C179H274N50O55S7/c1-87(2)65-111-155(261)208-112(66-93-33-15-13-16-34-93)146(252)195-77-133(239)225-139(88(3)4)172(278)213-117(71-136(244)245)158(264)199-100(44-31-62-190-178(186)187)144(250)193-75-131(237)198-102(40-22-27-58-181)148(254)219-124(167(273)205-109(56-64-285-12)145(251)194-76-132(238)197-101(39-21-26-57-180)147(253)200-105(43-25-30-61-184)151(257)220-123-81-286-288-83-125(221-152(258)106(203-166(123)272)45-32-63-191-179(188)189)169(275)210-113(68-95-46-48-97(234)49-47-95)156(262)206-110(177(283)284)50-53-129(185)235)82-287-291-86-128(168(274)202-104(42-24-29-60-183)150(256)212-116(70-135(242)243)159(265)207-111)224-175(281)142(91(9)10)228-164(270)121(79-231)216-157(263)115(69-96-74-192-99-38-20-19-37-98(96)99)211-170(276)126-84-289-290-85-127(171(277)217-122(80-232)165(271)227-141(90(7)8)174(280)218-120(78-230)163(269)201-103(41-23-28-59-182)149(255)204-108(154(260)222-126)52-55-134(240)241)223-160(266)118(72-137(246)247)214-173(279)140(89(5)6)226-162(268)119(73-138(248)249)215-176(282)143(92(11)233)229-161(267)114(67-94-35-17-14-18-36-94)209-153(259)107-51-54-130(236)196-107/h13-20,33-38,46-49,74,87-92,100-128,139-143,192,230-234H,21-32,39-45,50-73,75-86,180-184H2,1-12H3,(H2,185,235)(H,193,250)(H,194,251)(H,195,252)(H,196,236)(H,197,238)(H,198,237)(H,199,264)(H,200,253)(H,201,269)(H,202,274)(H,203,272)(H,204,255)(H,205,273)(H,206,262)(H,207,265)(H,208,261)(H,209,259)(H,210,275)(H,211,276)(H,212,256)(H,213,278)(H,214,279)(H,215,282)(H,216,263)(H,217,277)(H,218,280)(H,219,254)(H,220,257)(H,221,258)(H,222,260)(H,223,266)(H,224,281)(H,225,239)(H,226,268)(H,227,271)(H,228,270)(H,229,267)(H,240,241)(H,242,243)(H,244,245)(H,246,247)(H,248,249)(H,283,284)(H4,186,187,190)(H4,188,189,191)

InChI key

VDNVVLOBNHIMQA-UHFFFAOYSA-N

General description

Recombinant Buthus tamulus iberiotoxin expressed in E. coli. A single polypeptide chain of 37 amino acids that displays about 68% sequence homology with charybdotoxin (ChTx). Acts as a selective, reversible blocker of high conductance, Ca2+-activated K+ channels (PK,Ca). rIbTx does not block other types of voltage-dependent ion channels, especially other types of K+ channels that are sensitive to inhibition by ChTx. Shown to block PK,Ca in excised membrane patches from bovine aortic smooth muscle, acting exclusively at the outer face of the channel (IC50 = 250 pM). Causes a decrease in probability of channel opening and the channel open mean time. rIbTx appears to modulate ChTx binding by an allosteric mechanism.
Recombinant Buthus tamulus iberiotoxin expressed in E. coli. Acts as a selective, reversible blocker of high conductance Ca2+-activated K+ channels (IC50 = 250 pM).

Biochem/physiol Actions

Cell permeable: no
Primary Target
High conductance Ca2+-activated K+ channels
Product does not compete with ATP.
Reversible: yes
Target IC50: 250 pM against high conductance Ca2+-activated K+ channels
potently inhibits Ca2+-activated K+ channels expressed in Xenopus oocytes

Warning

Toxicity: Harmful (C)

Sequence

Pyr-Phe-Thr-Asp-Val-Asp-Cys⁷-Ser-Val-Ser-Lys-Glu-Cys¹³-Trp-Ser-Val-Cys¹⁷-Lys-Asp-Leu-Phe-Gly-Val-Asp-Arg-Gly-Lys-Cys²⁸-Met-Gly-Lys-Lys-Cys³³-Arg-Cys³⁵-Tyr-Gln-OH (disulfide bonds: 7 →28; 13 → 35; 17 → 33)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 3 months at -20°C or up to 2 weeks at 4°C.
Reconstitute in Tris-buffered saline (100 mM NaCl, 10 mM Tris-HCl, pH 7.5) containing 0.1% BSA.

Other Notes

Vanelli, G., et al. 1994. J. Appl. Physiol.76, 1098.
Garcia, M.L., et al. 1991. J. Bioeng. Biomembr.23, 615.
Galvez, A., et al. 1990. J. Biol. Chem. 265, 11083.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H300 + H310 + H330

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

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Hong-Bo Zhao et al.
Communications biology, 4(1), 24-24 (2021-01-06)
Inner hair cell (IHC) ribbon synapses are the first synapse in the auditory system and can be degenerated by noise and aging, thereby leading to hidden hearing loss (HHL) and other hearing disorders. However, the mechanism underlying this cochlear synaptopathy

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