biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
secondary antibodies
clone
monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(ELISA (1:5000)
Enzyme Immunoassay (1:5000))
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG2 Fc fragment.
This Mouse Anti-Human IgG₂, Fc Fragment Specific Alk-Phos Conjugate is validated for use in ELISA, Enzyme Immunoassay for the detection of Human IgG₂, Fc Fragment Specific.
Application
ELISA (≥1:5000)
Enzyme Immunoassay (1:5000)
Enzyme Immunoassay (1:5000)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM Tris-HCl, 1 mM MgCl₂, 50% glycerol, pH 8.0.
Other Notes
p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Monospecific for human IgG2 Fc fragment as determined by ELISA against normal human serum. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable