Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG₂ Fc fragment.

This Mouse Anti-Human IgG₂, Fc Fragment Specific Alk-Phos Conjugate is validated for use in ELISA, Enzyme Immunoassay for the detection of Human IgG₂, Fc Fragment Specific.

ELISA (≥1:5000)

Enzyme Immunoassay (1:5000)

Please refer to vial label for lot-specific concentration.

In 50 mM Tris-HCl, 1 mM MgCl₂, 50% glycerol, pH 8.0.

p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl₂ at 25°C, was used as substrate for testing immunoenzyme reactivity. Monospecific for human IgG₂ Fc fragment as determined by ELISA against normal human serum. Variables associated with assay conditions will dictate the proper working dilution.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)