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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source

rat

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PS/2, monoclonal

species reactivity

human, mouse

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2bκ

NCBI accession no.

NM_000885.4

UniProt accession no.

P13612

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGA4(3676)
mouse ... Itga4(16401)

General description

Virtually all lymphoid and myeloid cells in bone marrow are reactive with the anti-VLA-4 antibody. VLA-4 is absent or minimally detectable on stromal cells. Myeloid cells maintained in Dexter-type long-term cultures of murine bone marrow are positive, as are lymphoid cells from Whitlock-White cultures.

Application

Anti-Integrin α4 Antibody, clone PS/2 detects level of Integrin α4 & has been published & validated for use in FC, IF, IP, IH.
Flow cytometry: 2 μg per 106 cells

Immunohistochemistry on frozen tissue sections

Immunoprecipitation

Cell adhesion assays

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Biochem/physiol Actions

Clone PS/2 recognises the murine alpha4 chain of the VLA-4 integrin heterodimer, 150 kDa. The antibody also reacts with human VLA-4.
Reactivity with other species has not been confirmed.

Physical form

Format: Purified
Purified rat IgG2bκ in 0.01 M phosphate buffer, pH 7.1 containing no preservatives.

Preparation Note

Maintain at -20ºC for up to 12 months from date of receipt.Upon first use, aliquot into smaller quantities to avoid additional freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1131

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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