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Merck
CN

CBL194F

Anti-Cytokeratin 7 Antibody, clone LP5K, FITC conjugated

clone LP5K, Chemicon®, from mouse

Synonym(s):

Cytokeratin 7 Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Cytokeratin 7 Antibody, clone LP5K, FITC conjugated, clone LP5K, Chemicon®, from mouse

antibody product type

primary antibodies

biological source

mouse

conjugate

FITC conjugate

antibody form

saturated ammonium sulfate (SAS) precipitated

clone

LP5K, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG2b

NCBI accession no.

NM_005556.3

UniProt accession no.

P08729

shipped in

ambient

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... KRT7(3855)

Related Categories

Analysis Note

Tested

Application

Detect Cytokeratin 7 using this Anti-Cytokeratin 7 Antibody, clone LP5K, FITC conjugated validated for use in IC, IH, IH(P), FC.
Immunohistochemistry: Suitable for staining frozen sections and paraffin wax embedded tissue. Protease treatment of paraffin embedded sections is required prior to antibody application. Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).

Immunocytochemistry: isolated EVT cells fixed with 0.5% PFA at 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).

Flow cytometry: 10μL of conjugate per test.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Cytokeratins

Biochem/physiol Actions

The antibody reacts with cytokeratin 7 which has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed.

FUSION PARTNER: Sp2/0

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Cytoskeletal proteins from cultured HeLa cells

Other Notes

Please refer to lot specific datasheet.

Physical form

The antibody is ammonium sulphate precipitation followed by ion exchange chromatography. The conjugate is supplied in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin.

Preparation Note

Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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