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Merck
CN

CBL511

Anti-CD55 Antibody, clone BRIC 216

From mouse

Synonym(s):

DAF

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
BRIC 216, monoclonal
Species reactivity:
human
Application:
FACS, IHC (p), IP, WB
Citations:
5

Key Documents

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Product Name

Anti-CD55 Antibody, clone BRIC 216, clone BRIC 216, Chemicon®, from mouse

biological source

mouse

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

BRIC 216, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

NM_000574.2

UniProt accession no.

P08174

shipped in

wet ice

Quality Level

100

Gene Information

human ... CD55(1604)

Related Categories

Immunogen

Human fibroblast cell line

Application

Research Sub Category
Immunoglobulins & Immunology
Western blot: Non-reduced erythrocyte membrane prep; antigen is sialidase and trypsin resistant, pronase, chymotrypsin and AET sensitive
Flow Cytometry
Immunohistochemistry: Frozen and formalin-fixed paraffin-embedded sections. Protease antigen retreival is recommended.
Immunoprecipitation
Inhibition studies


Optimal working dilutions must be determined by the end user.
Anti-CD55 Antibody, clone BRIC 216 is an antibody against CD55 for use in FC, IP, WB, IH, IH(P).
Research Category
Inflammation & Immunology

Biochem/physiol Actions

This antibody recognizes the CD55 antigen a 70 kDa glycoprotein also known as Decay Accelerating Factor (DAF). The antigen is present on erthrocytes and other circulating blood cells and also on cells in non-haemopoietic tissue particularly epithelium and endothelium. It is found specifically at the placental fetal-maternal interface.

Physical form

Format: Purified
Protein G Purified
The immunoglobulin was purified from cell culture supernatant by affinity chromatography using protein G and is presented in 1 M Tris-buffered saline, pH 7.5, containing no preservatives. The antibody has been sterile-filtered.

Preparation Note

Aliquot antibody into small volumes and store at -20°C for up to 12 months from the date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy.
Holmes, C H, et al.
Journal of immunology (Baltimore, Md. : 1950), 144, 3099-3105 (1990)
Mapping of epitopes, glycosylation sites, and complement regulatory domains in human decay accelerating factor.
Coyne, K E, et al.
Journal of immunology (Baltimore, Md. : 1950), 149, 2906-2913 (1992)
Kyle A Carver et al.
Investigative ophthalmology & visual science, 57(2), 360-371 (2016-02-05)
Oxidative stress is a major factor involved in retinal pigment epithelium (RPE) apoptosis that underlies AMD. Drusen, extracellular lipid- and protein-containing deposits, are strongly associated with the development of AMD. Cell-derived microparticles (MPs) are small membrane-bound vesicles shed from cells.

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