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Merck
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FCMAB176F

Milli-Mark Anti-CD8 -FITC Antibody, clone DK25

clone DK25, Milli-Mark®, from mouse

Synonym(s):

Anti-CD8 FITC, Clone DK25 Anti-CD8

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44
eCl@ss:
32160702

Key Documents

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Product Name

Milli-Mark Anti-CD8 -FITC Antibody, clone DK25, clone DK25, Milli-Mark®, from mouse

biological source

mouse

conjugate

FITC conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

DK25, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1κ

NCBI accession no.

NP_001759.3

UniProt accession no.

P01732

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CD8A(925)

Related Categories

Analysis Note

Control
Human Lympnocytes.
Evaluated by flow cytomatry using human lymphocytes.

Application

Milli-Mark Anti-CD8 -FITC Antibody, clone DK25 detects level of CD8 & has been published & validated for use in FC.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Biochem/physiol Actions

Antibody recognizes Human CD8 antigen.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

68 kDa (Nakuchi, 1997)
CD8 is a 68 kDa disulphide-linked, transmembrane glycoprotein. The CD8 molecule serves as a receptor for MHC class I molecules and can mediate a function as a coreceptor in TCR-ligand binding and T-cell activation. Its cytoplasmic part is associated with the p56Ick tyrosine kinase (Nakuchi, 1997). CD8 is expressed by the great majority of cortical thymocytes and approximately 30% of medullary thymocytes, and by class I major histocompatibility complex restricted, mature suppressor/cytotoxic T cells. In addition a proportion of gamma delta T cells and NK cells express CD8 (Nakuchi, 1997). Thus, CD8 is expressed by about 25-35% of peripheral T cells, and about 30% of NK cells express low levels of CD8 (Leong, 1999). The specificity of Anti-CD8, DK25, is equivalent to that of the CD8-clustered antibodies OKT8 and Leu-2a (Stein, 1984).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified
Purified Mouse monoclonal IgG1k conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2

Preparation Note

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nicole Marzan-Rivera et al.
iScience, 25(8), 104764-104764 (2022-08-20)
The link between CD4+ T and B cells during immune responses to DENV and ZIKV and their roles in cross-protection during heterologous infection is an active area of research. Here we used CD4+ lymphocyte depletions to dissect the impact of
Kim J Hasenkrug et al.
mBio, 12(4), e0150321-e0150321 (2021-07-28)
Severe coronavirus disease 2019 (COVID-19) has been associated with T cell lymphopenia, but no causal effect of T cell deficiency on disease severity has been established. To investigate the specific role of T cells in recovery from severe acute respiratory
Shu-Qi Zhang et al.
Science translational medicine, 8(341), 341ra77-341ra77 (2016-06-03)
T cells recognize and kill a myriad of pathogen-infected or cancer cells using a diverse set of T cell receptors (TCRs). The affinity of TCR to cognate antigen is of high interest in adoptive T cell transfer immunotherapy and antigen-specific

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