Key Documents

View All Documentation

MAB10753

Anti-Caspase3 (active form) Antibody, clone 3D9.3

Name: Anti-Caspase3 (active form) Antibody, clone 3D9.3
Brand: MM

clone 3D9.3, from mouse

Synonym(s):

Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12

Select a Size

Change View

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

3D9.3, monoclonal

Application:

FACS, ICC, WB

Citations:

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3D9.3, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable

isotype

IgMκ

NCBI accession no.

NP_004337

UniProt accession no.

P42574

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CASP3(836)

Description

General description

Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.

~17 kDa observed. Caspase3 is cleaved during apoptosis into 17 kDa and 12 kDa active forms.

Immunogen

KLH-conjugated linear peptide corresponding to human Caspase3 (active form).

Application

Anti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Caspase3 (active form) in Hela and Hela/staurosporine 3 hr cells.
Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected Caspase3 (active form) in Jurkat T cells.

Biochem/physiol Actions

This antibody demonstrates specificity for the cleaved 17 kDa active form, but does not cross-react with the non-active (pro) form Caspase3 at 35 kDa.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Caspase3 (active form) in 10 µg of HeLa and staurosporine treated HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1090; 04-439

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c