Anti-Integrin β4 Antibody, clone ASC-8

Human beta4 integrin (CD104)

Human squamous cell carcinoma cells (SCC9)

Immunoprecipitation: A representative lot of this antibody clone precipitated PAGE bands of 66, 76, 120, 135 and 200 kDa (reduced) or 66, 110, 125, 130 and 190 kDa (non-reduced) from surface-biotinylated SCC9 cells.

Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue).

Flow Cytometry: A representative lot of this antibody clone stained human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

Functional Activity Assay: A representative lot of this antibody clone induced a sub-basal split at the basement membrane in non-wounded tissue. In addition, wound closure was significantly inhibited by ASC-8, as the epithelial tongue only covered 40 percent of the wound area at 120 hours post-wounding. These results demonstrate that the β4 adhesion-blocking antibody may have adverse effects on normal tissue (Egles, C., et al., 2010).

Working dilutions must be determined by end user.

Anti-Integrin β4 Antibody, clone ASC-8 is an antibody against Integrin β4 for use in FC, IP, IH.

Research Category
Cell Structure

Research Sub Category
Integrins

Flow Cytometry Analysis:
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 A431 cells.
0.5 µg of the antibody was used to detect Integrin β4 in 1x10^6 HeLa cells.

Replaces: 05-283

Format: Purified

Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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