MAB3222
Anti-BrdU Antibody, clone IIB5
clone IIB5, Chemicon®, from mouse
Synonym(s):
BrdU
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
IIB5, monoclonal
Technique(s):
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin)
Application:
FACS, ICC, IHC
Citations:
17
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
IIB5, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin)
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
General description
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Immunogen
A fusion of mouse myeloma cells with spleen cells from a mouse intraperitoneally immunized with BrdU conjugated to bovine serum albumin.
Application
Anti-BrdU Antibody, clone IIB5 is a Mouse Monoclonal Antibody for detection of Bromodeoxyuridine also known as BrdU & has been validated in FC, ICC, IHC(P).
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissues with acid/proteinase treatments. See:
http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.
Flow Cytometry: 1:100-1:200; acid methanol treatment required.
Optimal working dilutions must be determined by the end user.
In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)
http://www.protocol-online.org/prot/Immunology/Immunohistochemistry. For examples of various protocols.
Flow Cytometry: 1:100-1:200; acid methanol treatment required.
Optimal working dilutions must be determined by the end user.
In order for the antibody to work a pretreatment to expose DNA will be needed (1 mg/mL of pepsin in 0.01 M HCL 30 min at r/t)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Biochem/physiol Actions
Reacts specifically with bromodeoxyuridine. It also recognizes BrdU when incorporated into nuclear DNA.
Physical form
Format: Purified
Protein A Purified mouse immunoglobulin in PBS with 0.09% sodium azide.
Protein A purified
Preparation Note
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
After incorporation of BrdU, all DNA containing species
After incorporation of BrdU, all DNA containing species
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Dehuan Wang et al.
Research (Washington, D.C.), 7, 0433-0433 (2024-08-02)
Mitophagy maintains tissue homeostasis by self-eliminating defective mitochondria through autophagy. How mitophagy regulates stem cell activity during hair regeneration remains unclear. Here, we found that mitophagy promotes the proliferation of hair germ (HG) cells by regulating glutathione (GSH) metabolism. First
Laurence Faucher-Giguère et al.
NAR cancer, 4(1), zcab050-zcab050 (2022-01-21)
Small nucleolar RNAs (snoRNAs) are an omnipresent class of non-coding RNAs involved in the modification and processing of ribosomal RNA (rRNA). As snoRNAs are required for ribosome production, the increase of which is a hallmark of cancer development, their expression
Alyssa M Molinaro et al.
EMBO reports, 22(3), e50292-e50292 (2021-01-30)
Epimorphic regeneration commonly relies on the activation of reserved stem cells to drive new cell production. The planarian Schmidtea mediterranea is among the best regenerators in nature, thanks to its large population of adult stem cells, called neoblasts. While neoblasts
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB3222 | 04053252666414 |