Anti-Stage-Specific Embryonic Antigen-3 Antibody, clone MC-631

Stage-specific embryonic antigen 3 (SSEA-3) is a glycosphingolipid composed of five carbohydrate units connected to a sphingolipid. Sphingolipids are key players in cell signaling and the SSEA-3 is a well known marker of stem cells (ESCs, MSCs, iPSCs, CSCs) differentiation, where SSEA-3 rapidly disappears when the cells start to differentiate.

4-8 cell stage mouse embryos.

Immunocytochemistry Analysis: 10µg/mL from a representative lot detected Stage-Specific Embryonic Antigen-3 in H9 cells.
Immunocytochemistry Analysis: A representative lot detected SSEA-3 immunoreactivity among T
cell-derived iPSCs (TiPSCs) by fluorescent immunocytochemistry (Kishino, Y., et al. (2014). PLoS One. 9(5):e97397).
Immunocytochemistry Analysis: A representative lot detected the presence of SSEA-3-positive cancer stem cells (CSCs) among cultured HCT116 colorectal cancer (CRC) cells by fluorescent immunocytochemistry (Suzuki, Y., et al. (2013). Int. J. Oncol. 42(1):161-167).
Immunocytochemistry Analysis: A representative lot detected SSEA-3 immunoreactivity among induced pluripotent stem (iPS) cells from human molars-derived mesenchymal stromal cells (MSCs) by fluorescent immunocytochemistry (Oda, Y., et al. (2010). J. Biol. Chem. 285(38):29270-29278).
Flow Cytometry Analysis: A representative lot detected the presence of SSEA-3-positive cancer stem cells (CSCs) in five cultured colorectal cancer (CRC) cell lines, HCT116, Caco-2, DLD-1, HT-29, and SW480 (Suzuki, Y., et al. (2013). Int. J. Oncol. 42(1):161-167).
Immunofluorescence Analysis: A representative lot detected a mall number of SSEA-3-positive stromal cells in normal colorectal epithelia and SSEA-3-positive cancers in colorectal adenocarcinomas by fluorescent immunohistochemistry using frozen tissue sections (Suzuki, Y., et al. (2013). Int. J. Oncol. 42(1):161-167).

Research Category
Stem Cell Research

Research Sub Category
Cell Cycle, DNA Replication & Repair

This Anti-Stage-Specific Embryonic Antigen-3 Antibody, clone MC-631 is validated for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence and Immunohistochemistry for the detection of Stage-Specific Embryonic Antigen-3.

Reacts with the Stage-specific embryonic antigen-3 (SSEA-3) that is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression.

Format: Purified

Purified rat monoclonal IgMκ antibody in PBS with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Flow Cytometry in H9 cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected Stage-Specific Embryonic Antigen-3 in H9 cells.

Concentration: Please refer to lot specific datasheet.

Replaces: MAB4303

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.