MABC580
Anti-Clathrin Heavy Chain Antibody, clone 6F10.1
clone 6F10.1, from mouse
Synonym(s):
Clathrin heavy chain 1, Clathrin Heavy Chain, Clathrin heavy chain on chromosome 17, CLH-17
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
6F10.1, monoclonal
Application:
FACS, ICC, WB
Citations:
2
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
6F10.1, monoclonal
species reactivity
human, mouse, rat
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CLTC(1213)
General description
Clathrin heavy chain 1 (UniProt Q00610; also known as Clathrin heavy chain on chromosome 17, Clathrin heavy polypeptide (Hc), Clathrin heavy polypeptide-like 2, CLH-17) is encoded by the CLTC (also known as CHC, CLH17, CLTCL2, KIAA0034) gene in human (Entrez Gene ID 1213). Clathrin is the major protein component of the polyhedral coat of coated pits and vesicles. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of various macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
~190 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to human Clathrin Heavy Chain near the C-terminus.
Application
Anti-Clathrin Heavy Chain Antibody, clone 6F10.1 is an antibody against Clathrin Heavy Chain for use in Western Blotting, Flow Cytometry, Immunocytochemistry.
Flow Cytometry Analysis: 1.5 µg of this antibody from a representative lot detected Clathrin Heavy Chain in 1X10E6 Jurkat cells.
Immunocytochemistry Analysis: 25.0 µg/mL from a representative lot detected Clathrin Heavy Chain in HeLa, A431, HUVEC, and NIH/3T3 cells.
Immunocytochemistry Analysis: 25.0 µg/mL from a representative lot detected Clathrin Heavy Chain in HeLa, A431, HUVEC, and NIH/3T3 cells.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in PC12 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Clathrin Heavy Chain in 10 µg of PC12 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Clathrin Heavy Chain in 10 µg of PC12 cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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