123 kDa calculated

The LIF receptor is critical in modern mouse stem cell maintenance as the receptor for LIF, the embryonic stem cell (ESC) growth factor that maintains pluripotency and self renewal in mouse ES cells, however physiologically, the importance of LIF-R in real mouse development has been less clear. LIF receptor does play a signaling role and when cleaved its extracellular portion can act as a decoy receptor and block exogenous LIF from performing its function on ES cells. LIF receptor is expressed in multiple tissues in the mouse during development from the liver to the lung and brain. LIF receptor also is important in immune responses and in the stimulation of the interferon response to viral infection. Also LIF receptor has recently been identified as a metastasis suppressor that functions through the Hippo-YAP pathway in humans.

Recombinant protein corresponding to mouse LIF Receptor.

Anti-LIF Receptor, clone 1C7 (Azide Free) is an antibody against LIF Receptor for use in FC, Inhibition.

Inhibition Assay Analysis: A representative lot detected LIF Receptor in LIF binding to JAR cells (Blanchard, F., et al. (1997) International Immunology. 9(12):1775-1784).
Inhibition Assay Analysis: A representative lot detected LIF Receptor in IL3 proliferative response in Ba/F3 cells (Pitard, V., et al. (1997) Journal of Immunological Methods. 205:177-190).

Research Category
Stem Cell Research

Research Sub Category
Cell Cycle, DNA Replication & Repair

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Flow Cytometry in CHO D1IgD2-mycDAF cells .

Flow Cytometry Analysis: 1.0 µg of this antibody detected LIF Receptor in CHO D1IgD2-mycDAF.

Concentration: Please refer to lot specific datasheet.

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