MABD90
Anti-HLA-DRB1 Antibody, clone DJ9
clone DJ9, from mouse
Synonym(s):
Major histocompatibility complex, class II, DRB1, DRB1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
DJ9, monoclonal
Application:
FACS, ICC, IHC, WB
Citations:
1
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
DJ9, monoclonal
species reactivity
human
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HLA-DRB1(3123)
General description
HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. HLA-DRB1 binds peptides derived from antigens that access the endocytic route of antigen presenting cells and presents them on the cell surface for recognition by the CD4 T-cells. Class II molecules are expressed in antigen presenting cells B lymphocytes, dendritic cells, macrophages. HLA-Drb1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. HLA-DRB1, clone DJ9, has been shown to be a relevant marker of bone marrow derived mesenchymal stem cells and their osteogenic derivatives (Andersen, D., et al., 2011).
The previously assigned protein identifier Q5Y7A7 has been merged into P01911. Full details can be found on the UniProt database.
The previously assigned protein identifier Q5Y7A7 has been merged into P01911. Full details can be found on the UniProt database.
~30 kDa observed
Immunogen
Whole hMSC-TERT Cells
Application
Detect HLA-DRB1 using this Anti-HLA-DRB1 Antibody, clone DJ9 validated for use in Western Blotting, ICC, IHC, Flow Cytometry.
Research Category
Inflammation & Immunology
Stem Cell Research
Inflammation & Immunology
Stem Cell Research
Research Sub Category
Immunological Signaling
Mesenchymal Stem Cells
Immunological Signaling
Mesenchymal Stem Cells
This antibody has been shown to work in Immunocytochemistry (Andersen, D., et al. (2011)). This antibody has been shown to work in Immunohistochemistry (Andersen, D., et al. (2011)). This antibody has been shown to work in Flow Cytometry (Andersen, D., et al. (2011)).
Biochem/physiol Actions
This antibody recognizes human HLA-DRB1.
Physical form
Format: Purified
Analysis Note
Control
Daudi cell lysates
Daudi cell lysates
Evaluated by Western Blot in Daudi cell lysates. Western Blot Analysis: A 1:100,000 dilution of this antibody detected HLA-DRB1 in Duadi cell lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Takamichi Miyazaki et al.
Genesis (New York, N.Y. : 2000), 52(1), 49-55 (2013-11-21)
Human pluripotent stem cells (hPSCs) have the potential for unlimited expansion and differentiation into cell types of all three germ layers. Cryopreservation is a key process for successful application of hPSCs. However, the current conventional method leads to poor recovery
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABD90 | 04053252698835 |