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MABF151C

Anti-mouse IFN gamma, APC, clone XMG1.2 Antibody

clone XMG1.2, from rat, Allophycocyanin conjugate

Synonym(s):

Interferon gamma (IFN-gamma), IFN gamma

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
Allophycocyanin conjugate
Clone:
XMG1.2, monoclonal
Application:
FACS
Citations:
-

Key Documents

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biological source

rat

conjugate

Allophycocyanin conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

XMG1.2, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable

isotype

IgG1κ

UniProt accession no.

P01580

target post-translational modification

unmodified

Quality Level

100

Gene Information

mouse ... Ifng(15978)

Related Categories

General description

The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells, which allow the cytokine to modulate innate immunity, when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein, e.g. by flow cytometry, as well as for quantitative analysis of the secreted protein by ELISA, when paired with an appropriate secondary antibody. This clone is also widely used for neutralization of the functional activity of IFN-g in a variety of assays.

Application

This rat monoclonal Anti-mouse IFN gamma Antibody, APC, clone XMG1.2, Cat. No. MABF1515 is validated for use in Flow Cytometry, for the detection of IFN gamma.

Analysis Note

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 μg from a representative lot detected IFN gamma in one million C57Bl/6 splenocytes.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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