Skip to Content
Merck
CN

Skip To

MABF2103

Sigma-Aldrich

Anti-FcRH5 Antibody, clone F25

clone F25, from mouse

Synonym(s):

Fc receptor-like protein 5, FcR-like protein 5, FcRL5, BXMAS1, Fc receptor homolog 5, Immune receptor translocation-associated protein 2, IRTA2, CD307e

Sign Into View Organizational & Contract Pricing

Select a Size

Select a Size

Change View

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

F25, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

technique(s)

ELISA: suitable
flow cytometry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABF2102SAB2109139MABF841
antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

-

species reactivity

human

clone

F25, monoclonal

clone

F56, monoclonal

clone

polyclonal

clone

11B6, monoclonal

Gene Information

human ... FCRL5(83416)

Gene Information

human ... FCRL5(83416)

Gene Information

human ... FCRL5(57396)

Gene Information

human ... FCGR2A(2212), FCGR2B(2213)

technique(s)

ELISA: suitable, western blot: suitable, flow cytometry: suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

western blot: 1 μg/mL

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

General description

106.44 kDa calculated.
Fc receptor-like protein 5 (UniProt: Q96RD9; also known as FcR-like protein 5, FcRL5, BXMAS1, Fc receptor homolog 5, FcRH5, Immune receptor translocation-associated protein 2, IRTA2, CD307e) is encoded by the FCRL5 (also known as FCRH5, IRTA2, UNQ503/PRO820) gene (Gene ID: 83416) in human. FcRH5 is a single-pass type I membrane protein that is expressed in marginal zone B-cells, immunoblasts, tonsillar germinal center centrocytes and in the intraepithelial and interfollicular regions of the tonsil. It is involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages. It is shown to regulate BCR signaling and binds aggregated IgG and engages several of its Ig-domains to bind IgG. It is shown to be overexpressed in many lymphoma cell lines and on hairy cell leukemia cells. Serum levels of soluble FcRH5 are reported to be elevated in patients with several types of B cell tumors. FcRH5 is synthesized with a signal peptide (aa 1-15), which is cleaved off in the mature form that has a long extracellular domain (aa 16-851), a short transmembrane domain (aa 852-872), and a cytoplasmic tail (aa 873-977) that contains immunoreceptor tyrosine-based inhibitor motifs (ITIM). (Ref.: Franco, A., et al. (2013). J. Immunol. 190(11); 5739-46; Ise, T., et al. (2007). Leukemia 21(1); 169-174).

Specificity

Clone F25 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.

Immunogen

Epitope: extracellular domain
Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.

Application

Anti-FcRH5, clone F25, Cat. No. MABF2103, is a mouse monoclonal antibody that detects Fc receptor-like protein 5 and has been tested for use in ELISA, Flow Cytometry, and Western Blotting.
Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).

Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).

ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).
Research Category
Inflammation & Immunology

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Flow Cytometry in SU-DHL-5 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected FcRH5 in one million SU-DHL-5 cells .

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Regulatory Information

新产品

  • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service