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MABF33

Anti-MHC class II (I-A/I-E) Antibody, clone M5/114

Name: Anti-MHC class II (I-A/I-E) Antibody, clone M5/114
Brand: MM

clone M5/114, from rat

Synonym(s):

CD74 molecule, major histocompatibility complex, class II invariant chain, Ia-associated invariant chain, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), gamma chain of class II antigens, Ia antigen-

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

M5/114, monoclonal

Application:

FACS, IHC, WB

Citations:

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Properties

biological source

rat

Quality Segment

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

M5/114, monoclonal

species reactivity

mouse

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_001036070

UniProt accession no.

P04441

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Cd74(16149)

Description

General description

MHC class II-associated invariant chain (I-A/I-E), also known as Ia antigen-associated invariant chain, are single-pass type II membrane glycoproteins containing a single thyroglobulin type-I domain. They are critical components in the processing of MHC class II antigen through forming a stabilizing complex following the alpha/beta heterodimer synthesis, and orchestrating complex trafficking away from the ER compartments in which class II peptide loading occurs.

~32 kDa observed.
There are 2 isoforms produced by alternative splicing: 32 kDa Isoform Long and 24 kDa Isoform Short.

Application

Detect MHC class II (I-A/I-E) using this Anti-MHC class II (I-A/I-E) Antibody, clone M5/114 validated for use in WB, FC, IH.

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC on spleen cells. (Bhattacharya, A., et al. (1981). The Journal of immunology. 127(6):2488-2496.)

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH on thymus tissue. (de Bont, E., et al. (1999). Int. Immunol. 11(8):1295-1306.)

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blot in mouse spleen tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected MHC class II (I-A/I-E) in 10 µg of mouse spleen tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c