Anti-CD117 (c-kit) Antibody (mouse), APC, clone ACK2

CD117, also called c-Kit, is a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.

Immunogen information for this antibody is proprietary information.

Research Category
Inflammation & Immunology

This rat monoclonal antibody Anti-CD117 (c-kit) (mouse), APC & clone ACK2 validated for use in western blotting;IHC;ICC;Flow Cytometry for the detection of CD117 (c-kit).

This antibody is reported to be cross-reactive with rat CD117 and is extensively published for use in this species.

Purified rat monoclonal antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 µg of this antibody detected CD117 in one million C57BI/6 bone marrow cells.

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