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MABF366

Sigma-Aldrich

Anti-CD11b Antibody (human/mouse), APC, clone M1/70

clone M1/70, 0.2 mg/mL, from rat

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Synonym(s):
Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD antigen CD11b
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

conjugate

Allophycocyanin conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

M1/70, monoclonal

species reactivity

mouse, human

concentration

0.2 mg/mL

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2bκ

UniProt accession no.

P05555
P11215

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGAM(3684)

Related Categories

General description

The M1/70 antibody reacts with human and mouse CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. The antibody is also reported to be cross-reactive for Rhesus macaque CD11b.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
This Rat Monoclonal Antibody Anti-CD11b (human/mouse), APC, clone M1/70, Cat. No. MABF366 is validated for use in IP, IHC, FC, IF for the detection of CD11b.

Physical form

Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Raquel Gil-Edo et al.
Pharmaceuticals (Basel, Switzerland), 16(6) (2023-06-28)
This work focuses on the development of thirteen benzylethylenearyl ureas and one carbamate. After the synthesis and purification of the compounds, we studied their antiproliferative action on cell lines, such as HEK-293, and cancer ones, such as HT-29, MCF-7 or
Karpagam Srinivasan et al.
Cell reports, 31(13), 107843-107843 (2020-07-02)
Damage-associated microglia (DAM) profiles observed in Alzheimer's disease (AD)-related mouse models reflect an activation state that could modulate AD risk or progression. To learn whether human AD microglia (HAM) display a similar profile, we develop a method for purifying cell
Exploring BenzylethoxyAryl Urea Scaffolds for Multitarget Immunomodulation Therapies.
Gil-Edo, et al.
International Journal of Molecular Sciences, 24 (2023)

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