MABF415
Anti-CD4 Antibody (mouse), clone GK1.5
clone GK1.5, 0.5 mg/mL, from rat
Synonym(s):
T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-CD4 Antibody (mouse), clone GK1.5, clone GK1.5, 0.5 mg/mL, from rat
Quality Level
biological source
rat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
GK1.5, monoclonal
species reactivity
mouse
concentration
0.5 mg/mL
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG2bκ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Cd4(12504)
Related Categories
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
This Rat Monoclonal Antibody Anti-CD4 (mouse), clone GK1.5, Cat. No. MABF415 is validated for use in Immunoprecipitation, Immunohistochemistry, Flow Cytometry for the detection of CD4.
Biochem/physiol Actions
M
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will “compete” for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.
Immunogen
Immunogen information for this antibody is proprietary information.
Physical form
Format: Purified
Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, pH 7.2, and up to 0.1% sodium azide.
Preparation Note
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Keer Sun et al.
Journal of virology, 84(10), 5007-5014 (2010-03-05)
Interleukin-10 (IL-10) is an important anti-inflammatory molecule that can cause immunosuppression and long-term pathogen persistence during chronic infection of mice with viruses such as lymphocytic choriomeningitis virus. However, its specific role in immunity to acute viral infections is not fully
Fernanda A Lima et al.
Clinical and vaccine immunology : CVI, 19(9), 1382-1392 (2012-07-05)
Streptococcus pneumoniae is a pathogen of great importance worldwide. We have previously described the efficacy of a nasal vaccine composed of the pneumococcal surface protein A and the whole-cell pertussis vaccine as an adjuvant against a pneumococcal invasive challenge in
Christopher Siatskas et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 20(7), 1349-1359 (2012-02-23)
Tolerance induction, and thus prevention of autoimmunity, is linked with the amount of self-antigen presented on thymic stroma. We describe that intrathymic (i.t.) delivery of the autoantigen, myelin oligodendrocyte glycoprotein (MOG), via a lentiviral vector (LV), led to tolerance induction
Chao-Chih Wu et al.
PloS one, 8(7), e69336-e69336 (2013-08-13)
The purpose of this study was to investigate the anti-tumor effect and potential mechanisms of i.p. hyperthermia in combination with α-galactosylceramide (α-GalCer) for the treatment of ovarian cancer. In this study, immuno-competent tumor models were established using murine ovarian cancer
Prevention of diabetes in nonobese diabetic mice mediated by CD1d-restricted nonclassical NKT cells.
Nadia Duarte et al.
Journal of immunology (Baltimore, Md. : 1950), 173(5), 3112-3118 (2004-08-24)
A role for regulatory lymphocytes has been demonstrated in the pathogenesis of type 1 diabetes in the NOD mouse but the nature of these cells is debated. CD1d-restricted NKT lymphocytes have been implicated in this process. Previous reports of reduced
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