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MABF433

Sigma-Aldrich

Anti-CD45.1 (mouse), APC-Cy7, clone A20 Antibody

clone A20, 0.2 mg/mL, from mouse

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Synonym(s):
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

APC-Cy7

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

A20, monoclonal

species reactivity

mouse

concentration

0.2 mg/mL

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2aκ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Ptprc(19264)

Related Categories

General description

The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

Application

Anti-CD45.1 (mouse), APC-Cy7, clone A20 | MABF433 is an antibody against CD45.1 for use in Immunoprecipitation, Immunohistochemistry, Flow Cytometry.

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 1 μg of this antibody detected CD45.1 in one million SJL splenocytes.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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