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Merck
CN

MABN1847

Sigma-Aldrich

Anti-Synapsin-1 Antibody, clone MEGS3-10A

clone MEGS3-10A, from mouse

Synonym(s):

Synapsin-1, Synapsin I

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MEGS3-10A, monoclonal

species reactivity

mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgMκ

NCBI accession no.

NP_038708.3

UniProt accession no.

O88935

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Syn1 (20964)

General description

Synapsin-1 (UniProt O88935; also known as Synapsin I) is encoded by the Syn1 (also known as Syn-1) gene (Gene ID 20964) in murine species. The synapins constitute a family of abundant neuronal phosphoproteins that regulate SV trafficking and neurotransmitter release at the pre-synaptic terminal. Three genes exisit in mammals, encoding altogether 11 synapsin members by alternative splicings (Synapsin Ia & Ib by SYN1, Synapsin IIa & IIb by SYN2, Synapsin IIIa through IIIf by SYN3), Syn III is the most precociously expressed isoform that has a role in the early phases of neural development and is downregulated in mature neurons. On the other hand, Syn I and Syn II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1–2 months of life. The NH2-terminal region is divided in A, B, and C domains and is highly conserved among synapsin isoforms. Synapsins are regulated by postransloational phosphorylations. Domain A contains PKA and CaMKI/IV phosphorylation sites, domain B contains MAPK/Erk phosphorylation sites, and domain C is phosphorylated by Src. The c-terminal regions contain spliced domains and diverge among synapsin isoforms (domain D in Syn Ia and Ib, domain G in Syn IIa and IIb, domain H in Syn IIa, and domain J in Syn IIIa), although they all bear proline-rich regions binding to several SH3-containing proteins and additional phosphorylation sites for CaMKII, MAPK/Erk, and cdk1/5.
~75 kDa observed.

Immunogen

Recombinant full-length mouse synapsin-1.

Application

Immunocytochemistry Analysis: The ascites prior to antibody purification detected Synapsin-1 in primary mouse cortical neurons (Courtesy of Professor Michael E. Greenberg, Harvard Medical School, Boston, MA).
Western Blotting Analysis: An 1:1000 dilution of the ascites prior to antibody purification detected a higher Synapsin-1 expression in brain lysate from a 60-day old (P60) mouse than from a 6-day old (P6) mouse (Courtesy of Professor Michael E. Greenberg, Harvard Medical School, Boston, MA).
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Physical form

Format: Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synapsin-1 in 10 µg of mouse brain tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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