Chromaffin granule amine transporter (UniProt: Q8R090; also known as Solute carrier family 18 member 1, Vesicular amine transporter 1, VMAT1, VAT1) is encoded by the Scl19a1 (also known as Vmat1) gene (Gene ID: 110877) in murine species. Vesicular monoamine transporters (VMATs) are involved in presynaptic storage and release of neurotransmitters. They are located in the presynaptic region and are involved in the transport of monoamines into storage vesicle, which are released upon arrival of an action potential. Two structurally related, but pharmacologically distinct, VMATs have been described (VMAT1 and VMAT2) that are encoded by separate genes. VMAT1 is a multi-pass membrane acidic glycoprotein that is found in both large dense-core vesicles and in small synaptic vesicles. It s both C- and N-terminal regions are located in the cytosolic side of the vesicle. VMAT1 is detected in adrenal medulla, and brain (at protein level). In brain, specifically found in the medulla oblongata, pons, prefrontal cortex, striatum, dentate gyrus and hippocampus. VMAT1 is shown to play a key role in survival of hippocampal neurons and may contribute to neurocognitive deficits observed in neuropsychiatric disorders. (Ref.: Multani, PK., et al. (2013). Neurosci. 232; 32-44).

Clone N440/21 specifically detects Vesicular monoamine transporter 1 (VMAT1) and does not show reactivity with VMAT2. It targets an epitope with in 93 amino acids from the N-terminal first lumenal region.

Epitope: extracellular domain

Recombinant fragment corresponding to 93 amino acids from the N-terminal first lumenal region of murine Vesicular amine transporter 1 (VMAT1).

Anti-VMAT1, clone N440 21, Cat. No. MABN2547, is a highly specific mouse monoclonal antibody that targets Vesicular Monoamine Transporter 1 and has been tested for use in Western Blotting.

Research Category
Neuroscience

Evaluated by Western Blotting in rat cerebellum tissue lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected VMAT1 in rat cerebellum tissue lysate.

~93 kDa observed; 56.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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