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Merck
CN

MABN713

Anti-CA9 Antibody, clone 2D3

ascites fluid, clone 2D3, from mouse

Synonym(s):

Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, Membrane antigen MN, Renal cell carcinoma-associated antigen G250, RCC-associated antigen G250, pMW1, CA9

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-CA9 Antibody, clone 2D3, ascites fluid, clone 2D3, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2D3, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

Q16790

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CA9(768)

Related Categories

Physical form

Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Analysis Note

Control
HeLa and A549 cell lysates.
Evaluated by Western Blotting in HeLa and A549 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CA9 in HeLa and A549 cell lysates.

Application

Anti-CA9 Antibody, clone 2D3 is a highly specific mouse monoclonal antibody, that targets Carbonic Anhydrase & has been tested in western blotting, IHC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CA9 in human stomach and colon tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CA9 in NTERA-2 cells.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CA9 (Carbonic anhydrase 9) is a single-pass type I membrane protein belonging to the alpha-carbonic anhydrase family. CA9 may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. CA9 is primarily expressed in carcinoma cell lines and is found on the surface microvilli and in the nucleus.
~50 kDa observed. Uncharacterized bands may appear in some lysate(s).

Immunogen

Purified recombinant fragment of human CA9 expressed in E. Coli.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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