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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
16F4.1, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
human ... FFAR3(2865)
General description
Free fatty acid receptor 3 (UniProt O14843; also known as G-protein coupled receptor 41) is encoded by the FFAR3 (also known as GPR41) gene (Gene ID 2865) in human. Free fatty acids (FFAs) act as both energy sources and signaling molecules via G-protein-coupled receptors (GPCRs) called FFARs (FFA receptors). FFAs are categorized by the length of their carbon chains into short-chain FAs (SCFAs; less than 6 carbons), medium-chain FAs (MCFAs; 6–12 carbons), and long-chain FAs (LCFAs; more than 12 carbons). MCFAs and LCFAs activate FFAR1 and FFAR4, whereas SCFAs activate FFAR3 and FFAR2. FFAR3 is an SCFA receptor that can be activated by propionate (C3), butyrate (C4), and valerate (C5). FFAR3 mediates PYY and GLP-1 secretion from endocrine and sympathetic outflow-dependent heart rate regulation and energy expenditure, while β-Hydroxybutyrate, a ketone body produced in the liver during starvation, decreases sympathetic outflow by antagonizing FFAR3. FFAR3 is shown to couple Gαi/o pathway signaling upon activation by propionate binding in primary murine pancreatic beta cells, resulting in an inhibition of glucose-dependent insulin secretion. FFAR3 is a 7-transmembrane (a.a. 20-40, 48-68, 89-111, 133-153, 179-199, 223-243, 259-279) GPCR, having a short N-terminal extracellular portion (a.a. 1-19) and a long C-terminal cytomplasmic tail (a.a. 280-346).
~41 kDa observed. 38.65 kDa calculated.
Immunogen
KLH-conjugated linear peptide corresponding to a C-terminal cytoplasmic domain sequence of human FFAR3/GPR41.
Application
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected FFAR3/GPR41 in human breast cancer tissue, as well as in normal liver, pancreas, and small intestine tissue sections.
This Anti-FFAR3 Antibody, clone 16F4.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of GPR41.
Biochem/physiol Actions
Clone 16F4.1 targets an epitope in the C-terminal cytoplasmic domain of human FFAR3/GPR41.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in human adipose tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected FFAR3/GPR41 in 10 µg of human adipose tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected FFAR3/GPR41 in 10 µg of human adipose tissue lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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