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Merck
CN

PICM01250

Millipore

Millicell® Standing Cell Culture Inserts

Synonym(s):

Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE, 0.4 µm, Millicell-CM, cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22

Key Documents

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General description

With Millicell® standing inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® standing inserts make it possible to study both sides of the cell monolayer.

Application

  • Cell Attachment
  • Cell Growth
  • Cell Differentiation
  • Immunocytochemistry

Features and Benefits

  • Promotes excellent cell growth and provides an exceptional opportunity for cell studies
  • Low protein-binding hydrophilic PTFE membrane is ideal for live cell viewing and immunofluorescent application

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tong-Dian Zhang et al.
Oxidative medicine and cellular longevity, 2020, 4569268-4569268 (2020-06-23)
Mono-(2-ethylhexyl) phthalate (MEHP) and genistein have been classified as endocrine-disrupting chemicals (EDCs) which interfere with the differentiation and development of the male reproductive system. However, how these two EDCs would affect fetal rat testis development at a low dose was
Ming Zeng et al.
Gene, 753, 144810-144810 (2020-05-30)
Meiotic entry and progression require dynamic regulation of germline gene expression. m6A on mRNAs and recognition by YTHDC2 has been known as post-transcriptional regulatory complex, but the roles of this regulator remain unclear for meiotic initiation and progression in female
Gravity in mammalian organ development: differentiation of cultured lung and pancreas rudiments during spaceflight.
Spooner, B S, et al.
The Journal of Experimental Zoology, 269, 212-222 (1994)
R Le Bouffant et al.
Human reproduction (Oxford, England), 25(10), 2579-2590 (2010-07-31)
The initiation of meiosis is crucial to fertility. While extensive studies in rodents have enhanced our understanding of this process, studies in human fetal ovary are lacking. We used RT-PCR and immunohistochemistry to investigate expression of meiotic factors in human
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane
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Protocols

Toluidine Blue Staining Protocol for Millicell® Inserts

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

Generating Organotypic Air-Liquid Interface (ALI) 3D Skin Cultures as an In-Vitro Model of the Human Epidermis

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

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