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Merck
CN

PITP01250

Millicell® 24 well standing inserts

pore size 3.0 μm, diam. 12 mm, translucent polycarbonate membrane, Tissue Culture (TC)-treated surface, sterile

Synonym(s):

cell culture inserts, permeable culture insert, tissue culture insert, tissue culture plate

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About This Item

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32011202
Material:
polystyrene housing, translucent polycarbonate membrane
Pore size:
3.0 μm
Sterility:
γ-irradiated, sterile
Binding type:
Tissue Culture (TC)-treated surface

Key Documents

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material

polystyrene housing, translucent polycarbonate membrane

sterility

γ-irradiated, sterile

feature

hydrophilic, 2x106 pores/cm2 (pore density), hydrophilic

packaging

pack of 50 (individually blister packaged)

manufacturer/tradename

Millicell®

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable, cell differentiation: suitable, cell migration: suitable

H

10.5 mm

diam.

12 mm

size

24 wells

surface area

0.6 cm2

binding type

Tissue Culture (TC)-treated surface

shipped in

ambient

Quality Level

400

working volume

0.4 mL

color

translucent membrane

matrix

Isopore™

pore size

3.0 μm

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General description

With Millicell® inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.

Application

  • Cell Migration and Invasion
  • Cell Attachment
  • Cell Growth & Differentiation
  • Immunocytochemistry
  • Drug transport & permeability

Legal Information

ISOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Toluidine Blue Staining Protocol for Millicell® Inserts

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

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