FLAG-SNAP-hTERT expressing HeLa Cell Line

Telomerase play a critical role in regulating proliferation and senescence in normal somatic cells as well as most cancer cells. In humans, the telomerase reverse transcriptase (hTERT) is a lowly expressed protein with only several hundred molecules per cell. The low expression has made it difficult to detect, purify and study TERT activity from the endogenous loci.

To facilitate reliable detection of the endogenous TERT protein, a FLAG-SNAP tag was fused to the N-terminus of the TERT locus in HeLa cells. The FLAG-SNAP tag does not impair the function of the endogenous hTERT protein. Efficient immunopurification (IP) of the active telomerase ribonucleoprotein complexes may be accomplished by using the well-characterized FLAG antibody. The SNAP tag may be used to visualize subcellular localization of the low abundant endogenous TERT protein.

Reference:
Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, and Cech TR (2016) A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biology 16: 231-248.

Research Category
Cell Culture

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Cancer Cells

• Each vial contains ≥1X10⁶ viable cells.
• Cells are tested negative for HPV-16, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are negative for mycoplasma contamination.
• Cells are positive for HPV-18 by PCR.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

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