Assay Ready Mouse HL-1 Cardiac Muscle Cell Line

This product contains cells sold under a consumable label license and which may not be propagated. For the equivalent expandable, bankable LX-2 cell line, please see <IS7_PDP/PFPLink_from_Module_content target="https://www.sigmaaldrich.com/US/en/product/mm/scc065">SCC065.</IS7_PDP/PFPLink_from_Module_content>

HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion.

The HL-1 line was derived from AT-1 subcutaneous tumor excised from an adult female C57BL/6J mouse. The parental AT-1 line was originally derived from an atrial tumor growing in a transgenic mouse in which expression of the SV40 large T-antigen was targeted to atrial cardiomyocytes via the atrial natriuretic factor (ANF) promoter.

References:
1. Claycomb, W.C., Lanson, N.A. Jr., Stallworth, B.S., Egeland, D.B., Delcarpio, J.B., Bahinski, A., Izzo, N.J. Jr. (1998). HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte. Proc. Natl. Acad. Sci. USA. 95(6): 2979-2984.
2. White, S. M., Constantin, P. E., & Claycomb, W. C. (2004). Cardiac physiology at the cellular level: use of cultured HL-1 cardiomyocytes for studies of cardiac muscle cell structure and function. American Journal of Physiology-Heart and Circulatory Physiology, 286(3), H823-H829.

HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels. **SCCAR10065 cells are non-expandable and packaged at high density to enable readiness for hepatic fibrosis and other relevant assays without propagation or passage.

HL-1 Cardiac Muscle Cell Line should be stored in liquid nitrogen.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.