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Merck
CN

18597

Triethylammonium bicarbonate buffer

1 M, suitable for HPLC, LiChropur™

Synonym(s):

Triethylammonium hydrogen carbonate buffer

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About This Item

CAS Number:
15715-58-9
Beilstein:
3624751
MDL number:
MFCD00466938
UNSPSC Code:
12161702
NACRES:
NA.21

Key Documents

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form

liquid

Quality Level

100

quality

LiChropur™

concentration

1 M

technique(s)

HPLC: suitable

impurities

≤0.05% non-volatile matter

pH

8.4-8.6 (20 °C in neat)

density

1.015-1.024

UV absorption

λ: 260 nm Amax: ≤0.04
λ: 270 nm Amax: ≤0.04
λ: 280 nm Amax: ≤0.03
λ: 290 nm Amax: ≤0.02
λ: 500 nm Amax: ≤0.02

storage temp.

2-8°C

SMILES string

OC(O)=O.CCN(CC)CC

InChI

1S/C6H15N.CH2O3/c1-4-7(5-2)6-3;2-1(3)4/h4-6H2,1-3H3;(H2,2,3,4)

InChI key

AFQIYTIJXGTIEY-UHFFFAOYSA-N

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General description

Triethylammonium bicarbonate buffer is a commonly used chromatographic eluent, volatile buffer, mobile phase and ion-pairing agent for the purification of nucleic acids and biomolecules.

Application

Buffer for use in ion-exchange chromatography and electrophoresis.
Triethylammonium bicarbonate (TEAB) buffer may be used for studying proteins or noncovalent protein macromolecular complexes under “nondenaturing” conditions by electrospray ionization.

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM6943
BCCM3237
BCCL9729
BCCL3256
BCCK7304

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Improved preparation of 2 M triethylammonium bicarbonate
Carlson M, et al.
Green Chemistry Letters and Reviews, 8(3-4), 37-39 (2015)
D Lemaire et al.
Analytical chemistry, 73(8), 1699-1706 (2001-05-08)
The use of triethylammonium bicarbonate (TEAB) solution in electrospray mass spectrometry proved to be a very efficient way for studying proteins or noncovalent protein complexes under "nondenaturing" conditions. The low charge states observed in the mass spectra improve the separation
Peng Zhao et al.
Autophagy, 16(12), 2180-2192 (2020-01-28)
In plants, macroautophagy/autophagy has mainly been associated with stress-related processes but how it impacts normal physiological and developmental processes remains largely unexplored. Pollen germination is the critical first step toward fertilization in flowering plants. It is metabolically demanding and relies

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