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Biological Buffers

Image of light blue biological buffer solution in beaker with pipette.

It’s important to protect the integrity of your biomolecules and reagents by choosing the right biological buffer systems. We offer an extensive portfolio of biological buffers for a wide variety of applications, including cell culture buffers, PCR buffers, HEPES buffers, and assay buffers.

Key features of our advanced buffer systems:

  • High-purity solutions available in a wide range of grades
  • Multiple and user-friendly packaging options
  • Assured solution stability with pH control
  • No interference with biological processes
  • Add critical salts and nutrients to cells and tissues
  • Scalable from early research to commercial applications
  • Customized blending

Our advanced buffering systems can bring phenomenal stability to your cell culture, polymerase chain reaction (PCR), drug screening, bioprocessing, purification, and final formulation-based applications. All our products can be scaled from early research to commercial applications.

Products

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Product Number
Product Name
Product Description
Pricing
D8537
Dulbecco′s Phosphate Buffered Saline
Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
P4417
Phosphate buffered saline
tablet
T1503
Trizma® base
Primary Standard and Buffer, ≥99.9% (titration), crystalline
H3375
HEPES
≥99.5% (titration)
P3813
Phosphate buffered saline
powder, pH 7.4, for preparing 1 L solutions
H4034
HEPES
BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
T6066
Trizma® base
BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)
R0278
RIPA Buffer
T3253
Trizma® hydrochloride
reagent grade, ≥99.0% (titration), crystalline
H0887
HEPES solution
1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
S5761
Sodium bicarbonate
powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture
806552
Phosphate Buffered Saline
pH 7.4, liquid, sterile-filtered, suitable for cell culture
M8250
MES hydrate
≥99.5% (titration)
M1254
MOPS
≥99.5% (titration)
D8662
Dulbecco′s Phosphate Buffered Saline
With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture
E5134
Ethylenediaminetetraacetic acid disodium salt dihydrate
suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)
252859
Tris(hydroxymethyl)aminomethane
ACS reagent, ≥99.8%
S0751
Sodium phosphate monobasic
ReagentPlus®, ≥99.0%
93283
Tris-EDTA buffer solution
BioUltra, for molecular biology, pH 8.0
806544
Phosphate Buffered Saline
pH 7.2, liquid, sterile-filtered, suitable for cell culture
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Related Product Resources

Download: Buffer Selection Guide
Flyer: pHast Pack Electrophoresis
Flyer: Tricine Buffer
Flyer: HEPES Buffer
Flyer: Trizma Buffer
Brochure: Biological Buffer

Article: Buffer Reference Center
Article: Polymerase Chain Reaction (PCR) Basics
Article: TAE and TBE Running Buffers Recipe & Video
Article: M-Clarity™ Program
Article: Buffer Calculator

Complete Workflow Solutions

We provide a comprehensive selection of high-quality biochemicals in a wide range of buffer grades and innovative, user-friendly packaging options. In addition to off-the-shelf products, we offer custom reagent manufacturing and customized blending.

Bioperformance Certified Buffers

Our superior BioPerformance Certified buffers are stringently tested and pre-qualified to expedite your bioprocessing workflows in critical life science applications, such as cell culture, molecular biology, and diagnostic manufacturing. This may include enzymatic assay of nuclease, protease, and phosphatase, cellular toxicity, or endotoxin and bioburden levels. Using a single high-quality buffer across multiple workflows ensures the reproducibility of your experiment.

pHast Pack™ Ready-to-Use Buffers

Trying to get the correct concentration and pH value in buffer preparations can be a tedious and time-consuming task. Our pHast Pack™ ready-to-use buffers are specially designed to simplify your work. Simply mix the precisely pre-weighed packets with distilled water for quick and reproducible results again and again. By saving time, effort and even shelf space, you can focus on more important areas of your research endeavors.

Need help making a buffer? Our buffer preparation calculator helps you to quickly calculate buffer molarity, mass, and volume.

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Culture media preparation for in vitro cell cultivation including preparation of different media formats, sterilization methods, cell growth and regulation supplements, media storage and use.
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Bulk and Custom Services
We offer an extensive, reliable supply of production materials in bench to bulk volumes. Our custom services include quality control, testing, documentation services, chemical packaging, contract manufacturing according to your specifications.

Highlights


HEPES induces lysosomes and autophagy in culture cells
HEPES induces lysosomes & autophagy in culture cells - a call for action
Discover how HEPES may influence cell culture experiments and what to be aware of in data interpretation.



Webinar: Getting Your Buffers Right: How to Control the pH of Cell Culture Medium
This webinar will explain the principles of good practice in controlling the acid-base balance of culture media. It will highlight those combinations of buffers that can result in poor pH control, non-intuitive outcomes and erroneous inferences. 

Slide 1 of 2

Buffer Selection Guide
Request your Buffer Selection Guide
The Biological Buffers Selection Guide can be a great reference piece to hang in your lab. Request your DIN A1 (23.4" x 33.1") poster.


pHast Pack™ ready-to-use buffers
Save time, effort, and shelf space with pHast Pack™ ready-to-use buffers. Our formulations are specially developed to simplify your work, and strictly tested to ensure exact concentrations and pH.

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