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HEPES buffered saline

Name: HEPES buffered saline
Brand: SIAL

BioUltra, Molecular Biology, 2× concentrate

Synonym(s):

HEPES Saline Buffer Solution

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About This Item

NACRES:

NA.25

PubChem Substance ID:

329757705

UNSPSC Code:

12161700

MDL number:

MFCD00006158

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Properties

grade

Molecular Biology

Quality Segment

200

product line

BioUltra

form

liquid

quality

2× concentrate

composition

dextrose, 2.0 g/L , HEPES, 10 g/L , KCl, 0.74 g/L , NaCl, 16 g/L , Na₂HPO₄.2H₂O, 0.27 g/L

concentration

2 ×

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.339

pH

7.2 (25 °C)

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm A_max: 0.06, λ: 280 nm A_max: 0.04

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Application

HEPES buffered saline has been used:

for the preparation of plasmid DNA
as a reagent to prepare transfection master mix for DNA construct vectors
to develop hydrogel precursor for cell leakage assay

Preparation Note

concentration (in H₂O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na₂HPO₄.2H₂O; 2.0 g/l dextrose

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c