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Merck
CN

51558

HEPES buffered saline

BioUltra, Molecular Biology, 2× concentrate

Synonym(s):

HEPES Saline Buffer Solution

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About This Item

NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
MDL number:
Technical Service
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grade

Molecular Biology

Quality Level

product line

BioUltra

form

liquid

quality

2× concentrate

composition

dextrose, 2.0 g/L , HEPES, 10 g/L , KCl, 0.74 g/L , NaCl, 16 g/L , Na2HPO4.2H2O, 0.27 g/L

concentration

2 ×

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.339

pH

7.2 (25 °C)

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.06, λ: 280 nm Amax: 0.04

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

General description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Application

HEPES buffered saline has been used:
  • for the preparation of plasmid DNA
  • as a reagent to prepare transfection master mix for DNA construct vectors
  • to develop hydrogel precursor for cell leakage assay

Preparation Note

concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose


Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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J. Sambrook et al.
Molecular Cloning: A Laboratory Manual, B11 (1989)
Calcium phosphate transfection.
Kingston RE, Chen CA, Okayama H.
Current Protocols in Cell Biology, 20, 20-20 (2003)
Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications (2015)



Global Trade Item Number

SKUGTIN
A-046-1ML04061833326114
PHR3846-100MG04065268836538
Y000084504061833805312
Y000087804061833796658
A0132-1G04061833326909
A0132-5G04061833326923
A0132-100G04061832088488
A0132-25G04061833326916
51558-50ML04061832497006
109196002504022536937946