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Merck
CN

51558

HEPES buffered saline

BioUltra, Molecular Biology, 2× concentrate

Synonym(s):

HEPES Saline Buffer Solution

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About This Item

NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
MDL number:
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InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

grade

Molecular Biology

product line

BioUltra

form

liquid

quality

2× concentrate

composition

dextrose, 2.0 g/L , HEPES, 10 g/L , KCl, 0.74 g/L , NaCl, 16 g/L , Na2HPO4.2H2O, 0.27 g/L

concentration

2 ×

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.339

pH

7.2 (25 °C)

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.06, λ: 280 nm Amax: 0.04

Quality Level

General description

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.

Application

HEPES buffered saline has been used:
  • for the preparation of plasmid DNA
  • as a reagent to prepare transfection master mix for DNA construct vectors
  • to develop hydrogel precursor for cell leakage assay

Preparation Note

concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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Calcium phosphate transfection.
Kingston RE, Chen CA, Okayama H.
Current Protocols in Cell Biology, 20, 20-20 (2003)
Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications (2015)
J. Sambrook et al.
Molecular Cloning: A Laboratory Manual, B11 (1989)
Attachment of microstructures to single bacteria by two-photon patterning of a protein based hydrogel
Hasselmann NF and Horn W
Biomedical Physics & Engineering Express, 4(3), 035004-035004 (2018)
Influence of HEPES buffer on the local pH and formation of surface layer during in vitro degradation tests of magnesium in DMEM
Dezfuli SN, et al.
Progress in Natural Science: Materials International, 24(5), 531-538 (2014)

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