Tris Acetate-EDTA buffer Concentrate

Name: Tris Acetate-EDTA buffer Concentrate
Brand: SIAL

for Northern and Southern blotting, solution

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161700

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Properties

grade

Molecular Biology

form

solution

quality

50x Concentrate

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

pH

8.3±0.2 (25 °C)

density

1.09 g/mL at 20 °C

storage temp.

room temp

Description

General description

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.

Application

Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.