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Merck
CN

67996

Tris Acetate-EDTA buffer Concentrate

for Northern and Southern blotting, solution

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

Key Documents

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grade

Molecular Biology

form

solution

quality

50x Concentrate

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

pH

8.3±0.2 (25 °C)

density

1.09 g/mL at 20 °C

storage temp.

room temp

Related Categories

General description

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.

Application

Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.

Preparation Note

The TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN4880
BCCL8658
BCCK7185
BCCH9646
BCCG8073

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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