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Sigma-Aldrich

Phosphate buffered saline

BioUltra, solution

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Synonym(s):
PBS
MDL number:
MFCD00131855
eCl@ss:
32129211
NACRES:
NA.25

product line

BioUltra

Quality Level

200

form

solution

impurities

insoluble matter, passes filter test

pH

7.1-7.3

cation traces

Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

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General description

Phosphate buffered saline is a non-toxic biological buffer generally containing sodium phosphate, sodium chloride, and potassium phosphate. It prevents cells from osmosis-mediated rupture or dehydration. PBS is widely used for washing cells, immunohistochemistry, and various other cellular techniques.

Application

Phosphate buffered saline has been used in sample preparation for quantifying the wound fluid proteins from pressure ulcers in patients with spinal cord injury (SCI). It has also been used for cell rinsing in various biological researches.

Physical form

150 mM sodium phosphate; 150 mM NaCl; pH 7.2±0.1 (25°C)

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Precautionary Statements

P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Laura E Edsberg et al.
The journal of spinal cord medicine, 38(4), 456-467 (2014-06-27)
To determine whether the biochemistry of chronic pressure ulcers differs between patients with and without chronic spinal cord injury (SCI) through measurement and comparison of the concentration of wound fluid inflammatory mediators, growth factors, cytokines, acute phase proteins, and proteases.
Marco L Leung et al.
Nature protocols, 11(2), 214-235 (2016-01-08)
Single-cell DNA sequencing methods are challenged by poor physical coverage, high technical error rates and low throughput. To address these issues, we developed a single-cell DNA sequencing protocol that combines flow-sorting of single nuclei, time-limited multiple-displacement amplification (MDA), low-input library
N C Martin et al.
Science & justice : journal of the Forensic Science Society, 46(3), 179-184 (2007-03-29)
In the forensic science laboratory, the recovery of spermatozoa from vaginal swabs, or vaginal cells from penile swabs, can help determine if sexual intercourse may have taken place. There are several methods used to recover spermatozoa and cells from the
Fernando Cid-Samper et al.
Cell reports, 25(12), 3422-3434 (2018-12-20)
Recent evidence indicates that specific RNAs promote the formation of ribonucleoprotein condensates by acting as scaffolds for RNA-binding proteins (RBPs). We systematically investigated RNA-RBP interaction networks to understand ribonucleoprotein assembly. We found that highly contacted RNAs are structured, have long UTRs
P Massari et al.
Methods in molecular medicine, 67, 587-597 (2001-01-01)
Microorganisms or microbial products have been shown to induce or protect cells from activation-induced cell death or apoptosis (1-3). Induction of apoptosis by some bacterial invaders, like shigella, might aid in spread of the organism (4), whereas inhibition of apoptosis
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